지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2005.1
- 수록면
- 447 - 456 (10page)
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초록· 키워드
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We investigated expression profiles and biological effects of the naked DNA vectors in the heart. To this end, naked DNA vector was injected into the apex of the beating rat heart after thorocotomy. When the expression of LacZ reporter was examined by reverse transcription-PCR and histochemical staining for β -galactosidase, LacZ expression was detected only in the heart, suggesting limited dissemination of the injected vector in vivo. Even within the heart, LacZ expression was limited to the injection area (apex). Similar observations were made with other transgenes such as VEGF and basic fibroblast growth factor (bFGF), where 77% and 69% of the total transgene exprssion were detected in the heart segments containing the apex. Although VEGF and bFGF expressions were de-tected until 2 weeks after DNA injection, the highest levels of VEGF and bFGF were observed on day 5 and day 1, respectively. The optimal doses of the vectors were 10 µg and 25 µg for the VEGF and bFGF vectors, respectively. Interestingly, injection of bFGF vector led to 50% increase in the level of endogenous murine VEGF expression. Consistent with this finding, the number of vessels that stained positive for α -smooth muscle actin was increased in the bFGF vector-injected heart. These results suggest that simple injection of naked DNA vector may be sufficient to induce significant angio-genesis in the myocardium and that naked DNA gene therapy may be a feasible approach for the treatment of ischemic heart disease.
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참고문헌 (28)
참고문헌 신청
/ 1995 / Basic fibroblast growth factor upregulates theexpression of vascular endothelial growth factor in vascularsmooth muscle cells 92
/ 1996 / Comparative effects of basic fibroblastgrowth factor and vascular endothelial growth factor oncoronary collateral development and the arterial response toinjury
/ 1996 / Direct intramusculargene transfer of naked DNA encoding vascularendothelial growth factor augments collateral developmentand tissue perfusio
/ 1997 / Hemodynamic effects of intracoronaryVEGF delivery evidence of tachyphylaxis and NO dependenceof response
/ 1998 / Biologic bypass with the use of adenovirus-mediatedgene transfer of the complementary deoxyribonucleic acidfor vascular endothelial growth factor 121 improves myoca
/ 1996 / Comparative effects of basic fibroblastgrowth factor and vascular endothelial growth factor oncoronary collateral development and the arterial response toinjury
/ 1996 / Direct intramusculargene transfer of naked DNA encoding vascularendothelial growth factor augments collateral developmentand tissue perfusio
/ 1997 / Hemodynamic effects of intracoronaryVEGF delivery evidence of tachyphylaxis and NO dependenceof response
/ 1998 / Biologic bypass with the use of adenovirus-mediatedgene transfer of the complementary deoxyribonucleic acidfor vascular endothelial growth factor 121 improves myoca
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