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논문 기본 정보
- 자료유형
- 학술저널
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제8호
- 발행연도
- 2008.1
- 수록면
- 1,401 - 1,407 (7page)
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초록· 키워드
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The roles of conserved amino acid residues (Val329-Ala330-
Asn331-Glu332), constituting an extra sugar-binding space
(ESBS) of Thermus maltogenic amylase (ThMA), were
investigated by combinatorial saturation mutagenesis.
Various ThMA mutants were firstly screened on the basis
of starch hydrolyzing activity and their enzymatic properties
were characterized in detail. Most of the ThMA variants
showed remarkable decreases in their hydrolyzing activity,
but their specificity against various substrates could be
altered by mutagenesis. Unexpectedly, mutant H-16 (Gly-
Leu-Val-Tyr) showed almost identical hydrolyzing and
transglycosylation activities to wild type, whereas K-33
(Ser-Gly-Asp-Glu) showed an extremely low transglycosylation
activity. Interestingly, K-33 produced glucose, maltose, and
acarviosine from acarbose, whereas ThMA hydrolyzed
acarbose to only glucose and acarviosine-glucose. These
results propose that the substrate specificity, hydrolysis
pattern, and transglycosylation activity of ThMA can be
modulated by combinatorial mutations near the ESBS.
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참고문헌 (21)
참고문헌 신청
Baek, J. S. / 2003 / Role of dipeptide at extra sugar-binding space of Thermus maltogenic amylase in transglycosylation activity. J. Microbiol / Biotechnol 13 : 969 ~ 975
CHO, KIL SOON / 2006 / Potential Suppression of Dental Caries by Maltosyl-Mannitol Produced byBacillus stearothermophilus Maltogenic Amylase / Journal of Microbiology and Bio
CHO, MEE-HYUN / 2007 / Extracellular Secretion of a Maltogenic Amylase from Lactobacillus gasseriATCC33323 in Lactococcus lactis MG1363 and its Application on theProduction o
Cha, H. J. / 1998 / Molecular and enzymatic characterization of a maltogenic amylase that hydrolyzes and transglycosylates acarbose. Eur / Biochem 253 : 251 ~ 262
Cheong, K. A. / 2002 / Catalytic activities of intracellular dimeric neopullulanase on cyclodextrin, acarbose and maltose. Biotechnol. Appl / Biochem 35 : 27 ~ 34
CHO, KIL SOON / 2006 / Potential Suppression of Dental Caries by Maltosyl-Mannitol Produced byBacillus stearothermophilus Maltogenic Amylase / Journal of Microbiology and Bio
CHO, MEE-HYUN / 2007 / Extracellular Secretion of a Maltogenic Amylase from Lactobacillus gasseriATCC33323 in Lactococcus lactis MG1363 and its Application on theProduction o
Cha, H. J. / 1998 / Molecular and enzymatic characterization of a maltogenic amylase that hydrolyzes and transglycosylates acarbose. Eur / Biochem 253 : 251 ~ 262
Cheong, K. A. / 2002 / Catalytic activities of intracellular dimeric neopullulanase on cyclodextrin, acarbose and maltose. Biotechnol. Appl / Biochem 35 : 27 ~ 34
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