지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제1호
- 발행연도
- 2005.1
- 수록면
- 35 - 39 (5page)
이용수
초록· 키워드
오류제보하기
To identify the activity of recombinant subtilisins(subtilisin BPN' and subtilisin Carlsberg), three differentzymography methods, SDS-fibrin zymography (SDS-FZ),reverse fibrin zymography (RFZ), and isoelectric focusingfibrinzymography (IEF-FZ), were used. The recombinantsubtilisins BPN' and Carlsberg did not migrate into theelectrophoretic field based on a Laemmli buffer system, insteadforming a "binding mode" at the top part of the separating gelswith the SDS-FZ and RFZ techniques. Yet, this problem wasresolved when using IEF-FZ with a pH range from 3 to 10. Inaddition, all these methods enabled the activity of a recombinantpro-subtilisin DJ-4 to be detected without a refolding pathway.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
A / 1996 / Evidence for intramolecularprocessing of prosubtilisin sequestered on a solid support 262 : 595 ~ 599
Brown / 1982 / Sensitive detection of amylolytic analyses of recombinant prosubtilisinDJ-4 and mature subtilisin DJ-4 All procedures were performed as described in Figs Fibri
Chang / 2000 / Potent fibrinolytic enzyme from a mutant of Bacillus subtilisIMR-NK1 48 : 3210 ~ 3216
Choi / 2000 / Two fibrin zymographymethods for analysis of plasminogen activators on gels 281 : 236 ~ 238
Choi / 2001 / Identification of total extracellular fibrinase from Bacillussp DJ using one- or two-dimensional fibrin zymography forproteomic approach 11 : 1111 ~ 1114
Brown / 1982 / Sensitive detection of amylolytic analyses of recombinant prosubtilisinDJ-4 and mature subtilisin DJ-4 All procedures were performed as described in Figs Fibri
Chang / 2000 / Potent fibrinolytic enzyme from a mutant of Bacillus subtilisIMR-NK1 48 : 3210 ~ 3216
Choi / 2000 / Two fibrin zymographymethods for analysis of plasminogen activators on gels 281 : 236 ~ 238
Choi / 2001 / Identification of total extracellular fibrinase from Bacillussp DJ using one- or two-dimensional fibrin zymography forproteomic approach 11 : 1111 ~ 1114
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Miniscale Identification and Characterization of Subtilisins from Bacillus sp. Strains
Journal of Microbiology and Biotechnology
2005 .01
-
Identification of Three Extracellular Proteases from Bacillus subtilis KCTC 3014
Journal of Microbiology and Biotechnology
2006 .01
-
Bacillus sp. CP-1 유래 subtilisin CP-1 생산에 있어 tryptic soy broth (TSB)와 Luria-Bertani (LB)배지가 미치는 영향 및 subtilisin CP-1의 특성
생명과학회지
2012 .06
-
Picosecond Protein Fluorescence and Time-Resolved $Eu^{3+}$ Luminescence Spectroscopic Studies on the Roles of $Ca^{2+}$ in Subtilisin Carlsberg
한국생물물리학회 학술발표회
1997 .01
-
Changes in Transcriptional Level of Subtilisin-like Proteases of Bacillus licheniformis during Fermentation of Fast-fermented Soybean Paste
한국미생물학회 학술대회논문집
2014 .04
-
Immobilization of Serine Protease Subtilisin Carlsberg on Pentynyl Dextran and Its Application for Transesterification of N-acetyl-L-phenyl Ethyl Ester
한국미생물학회 학술대회논문집
2012 .05
-
자이모그라피 기술의 문제점과 해결
생명과학회지
2019 .12
-
Purification and Characterization of a Keratinase from a Feather-Degrading Fungus, Aspergillus flavus Strain K-03
Mycobiology
2007 .01
-
Measurement of Fibrin Fiber Strength
한국진공학회 학술발표회초록집
2005 .08
-
일차-IEF(One-Dimensional Isoelectric Focusing)에 의한 한국인 HLA-A2 아형의 구분
The Journal of the Korean Society for Microbiology
1991 .02
-
A Novel Transglutaminase Substrate from Streptomyces mobaraensis Inhibiting Papain-Like Cysteine Proteases
Journal of Microbiology and Biotechnology
2011 .01
-
Correlation between pr1 and pr2 Gene Content and Virulence in Metarhizium anisopliae Strains
Journal of Microbiology and Biotechnology
2014 .01
-
Bacillus subtilis BK-17 유래 혈전용해 효소의 특성
생명과학회지
2005 .12
-
Immune Responses to Recombinant Antigen Delivered by Live Attenuated Recombinant Salmonella Vaccines
한국미생물학회 학술대회논문집
2001 .10
-
Characterization of an Antarctic alkaline protease, a cold-active enzyme for laundry detergents
미생물학회지
2018 .03
-
Production of Lipocortin-11-185 Using A Recombinant of Escherichia coli.
The Journal of Microbiology
1997 .06
-
EXPRESSION OF RECOMBINANT PROTEINS IN VARIOUS EXPRESSION SYSTEMS
한국식물학회 심포지움
1998 .01
-
인지활동이 정상여성노인의 뇌파에 미치는 영향 분석
Journal of The Korean Data Analysis Society
2009 .01
-
Fibrin 분해 효소-생성 Bacillus subtilis subsp. subtilis BYSnat3의 분리 및 특성
미생물학회지
2019 .12
이 논문의 저자 정보
최근 본 자료
댓글(0)
0