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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제3호
- 발행연도
- 2005.1
- 수록면
- 537 - 543 (7page)
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초록· 키워드
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Subtilisin (EC 3.4.21.14) is the major extracellularalkaline serine protease of Bacillus species. Previously, wefound that subtilisins did not migrate in the electrophoreticfield in the Laemmili buffer system due to their high pI values(over 8.8); however, it formed a “binding mode” at the topof the separating gel [5]. Utilizing this characteristic, foursubtilisins from Bacillus sp. strains (e.g., B. subtilis 168, B.subtilis KCTC 1021, B. amyloliquefaciens KCTC 3002, andBacillus sp. DJ-1 and DJ-4) were easily and quickly identifiedby an over-running electrophoretic technique with a miniscaleculture supernatant (less than 20 ml) without any columnchromatographic steps. Two subtilisins (DJ-1 and a recombinantversion) from Bacillus sp. DJ-1 were characterized, andthe enzymatic properties were determined by SDS-fibrinzymography and densitometric analysis. Based on thisobservation, the recombinant pro-subtilisin DJ-1 showed thesame “binding mode,” similar to native subtilisin DJ-1. Onthe other hand, mature subtilisin DJ-1 without pro-peptideshowed no enzymatic activity.
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참고문헌 (29)
참고문헌 신청
[학술지(정기
Bradford / 1976 / A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye binding : 248 ~ 254
Brown / 1982 / Sensitive detection of amylolytic : 164 ~ 172
Chang / 2000 / Potent fibrinolytic enzyme from a mutant of Bacillus subtilisIMR-NK1 48 : 3210 ~ 3216
Choi / 2000 / Two fibrin zymographymethods for analysis of plasminogen activators on gels 281 : 236 ~ 238
Bradford / 1976 / A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye binding : 248 ~ 254
Brown / 1982 / Sensitive detection of amylolytic : 164 ~ 172
Chang / 2000 / Potent fibrinolytic enzyme from a mutant of Bacillus subtilisIMR-NK1 48 : 3210 ~ 3216
Choi / 2000 / Two fibrin zymographymethods for analysis of plasminogen activators on gels 281 : 236 ~ 238
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Dissection of the Dimerization Modes in the DJ-1 Superfamily
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Cellular Responses of Pseudomonas sp. DJ-12 to the Stresses of Several Aromatic Pollutants
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Antibacterial Activities of Bacillus sp. Isolation from Soil
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Improvement of 4-Chlorobiphenyl Degradation by a Recombinant Strain, Pseudomonas sp. DJ12-C
The Journal of Microbiology
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Pseudomonas sp. Strain DJ77로 부터 Phenanthrene 분해 유전자군의 클로닝과 대장균에서의 발현
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Pseudomonas sp. Strain DJ77에서 phnF 유전자의 구조
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Crystal structures of human DJ-1 and Escherichia coli Hsp31 that share an evolutionarily conserved domain
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Production of Stress-shock Proteins in Pseudomonas sp DJ-12 Treated with 4-Hydroxybenzoate
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