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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제10호
- 발행연도
- 2015.1
- 수록면
- 1,702 - 1,708 (7page)
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We have developed a new shuttle plasmid, designated as pLK1-MCS that can replicate in both Clostridium acetobutylicum and Escherichia coli, by combining the pUB110 and pUC19 plasmids. Plasmid pLK1-MCS replicated more stably than previously reported plasmids containing either the pIM13 or the pAMβ1 replicon in the absence of antibiotic selective pressure. The transfer frequency of pLK1-MCS into C. acetobutylicum was similar to the transfer frequency of other shuttle plasmids. We complemented C. acetobutylicum ML1 (that does not produce solvents such as acetone, butanol, and ethanol owing to loss of the megaplasmid pSOL1 harboring the adhE1-ctfAB-adc operon) by introducing pLK1-MCS carrying the adhE1-ctfAB-adc operon into C. acetobutylicum ML1. The transformed cells were able to resume anaerobic solvent production, indicating that the new shuttle plasmid has the potential for practical use in microbial biotechnology.
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참고문헌 (23)
참고문헌 신청
Boe L / 1989 / Replication origins of single-stranded-DNA plasmid pUB110 / J. Bacteriol 171 : 3366 ~ 3372
Chopra I / 1973 / A variety of staphylococcal plasmids present as multiple copies / J. Gen. Microbiol 79 : 343 ~ 345
Clark SW / 1989 / Isolation and characterization of mutants of Clostridium acetobutylicum ATCC 824 deficient in acetoacetyl-coenzyme A: acetate/butyrate:coenzyme A-transferas
Cornillot E / 1997 / The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the stra
Durre P. / 2004 / Handbook on Clostridia / CRC Press : 659 ~ 699
Chopra I / 1973 / A variety of staphylococcal plasmids present as multiple copies / J. Gen. Microbiol 79 : 343 ~ 345
Clark SW / 1989 / Isolation and characterization of mutants of Clostridium acetobutylicum ATCC 824 deficient in acetoacetyl-coenzyme A: acetate/butyrate:coenzyme A-transferas
Cornillot E / 1997 / The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the stra
Durre P. / 2004 / Handbook on Clostridia / CRC Press : 659 ~ 699
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