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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제2호
- 발행연도
- 2006.1
- 수록면
- 205 - 211 (7page)
이용수
초록· 키워드
오류제보하기
A quantitative analytical method to detect new linesof.geneticaly modified (GM) maize, NK603 and TC1507,has been developed by using a real-time polymerase chainreaction (PCR). To detect these GM lines, two specific primerpairs and probes were designed. A plasmid as a referencemolecule was constructed from an endogenous DNA sequence(CaMV) 35S promoter used in most GMOs, and each DNAsequence specific to the NK603 and TC1507 lines. For thevalidation of this method, the test samples of 0, 0.1, 0.5, 1.0,3.0, 5.0, and 10.0% each of the NK603 and TC1507 GMmaize were quantitated. At the 3.0% level, the biases (meanvs. true value) for the NK603 and TC1507 lines were 3.3%and 15.7%, respectively, and their relative standard deviationswere 7.2% and 5.5%, respectively. These results indicate thatquantitatively detect the NK603 and TC1507 lines of GM maize.
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등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Ausubel, F. M / 1997 / Current Protocols in Molecular Biology, vol 2. Appendix 1B.1 / John Wiley & Sons
Clive, J / 2004 / Preview: Global status of commercialized biotech/GM crops 2004 / ISAAA (32)
Firko, M. J / 2001 / Apporval of Mycogen Seed c/o Dow Agrosciences LLC and Pioneer Hi-Bred International Inc. Request seeking a determination of non-regulated status for Bt cry1F insec
HEO, MUN-SEOK / 2004 / Detection of Genetically Modified Maize by Multiplex PCR Method / Journal of Microbiology and Biotechnology 14 (6) : 1150 ~ 1156
HWANG, OK-HWA / 2004 / Production of Recombinant Proteins asImmuno-Analytical Markers ofGenetically-Modified Organisms (GMO) / Journal of Microbiology and Biotechnology 14 (
Clive, J / 2004 / Preview: Global status of commercialized biotech/GM crops 2004 / ISAAA (32)
Firko, M. J / 2001 / Apporval of Mycogen Seed c/o Dow Agrosciences LLC and Pioneer Hi-Bred International Inc. Request seeking a determination of non-regulated status for Bt cry1F insec
HEO, MUN-SEOK / 2004 / Detection of Genetically Modified Maize by Multiplex PCR Method / Journal of Microbiology and Biotechnology 14 (6) : 1150 ~ 1156
HWANG, OK-HWA / 2004 / Production of Recombinant Proteins asImmuno-Analytical Markers ofGenetically-Modified Organisms (GMO) / Journal of Microbiology and Biotechnology 14 (
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