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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제3호
- 발행연도
- 2015.1
- 수록면
- 321 - 327 (7page)
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초록· 키워드
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Nocardia salmonicida is one of the main pathogens of fish nocardiosis. The purpose of this study was to build a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of N. salmonicida. A set of four primers were designed from the 16S-23S rRNA intergenic spacer region of N. salmonicida, and conditions for LAMP were optimized as incubating all the reagents for 60 min at 64°C. LAMP products were judged with agar gel electrophoresis as well as with the naked eye after the addition of SYBR Green I. Results showed the sensitivity of the LAMP assay was 1.68 × 103 CFU/ml (16.8 CFU per reaction) and 10-fold higher than that of PCR. The LAMP method was also effectively applied to detect N. salmonicida in diseased fish samples, and it may potentially facilitate the surveillance and early diagnosis of fish nocardiosis.
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참고문헌 (23)
참고문헌 신청
Biswas G / 2014 / Loop-mediated isothermal amplification (LAMP) assays for detection and identification of aquaculture pathogens: current state and perspectives / Appl. Micro
Cai SH / 2010 / Loop-mediated isothermal amplification method for rapid detection of Vibrio alginolyticus, the causative agent of vibriosis in mariculture fish / Lett. Appl.
Chen SC / 2000 / Nocardiosis in sea bass, Lateolabrax japonicus, in Taiwan / J. Fish Dis 23 : 299 ~ 307
Elkesh A / 2013 / Systemic nocardiosis in a Mediterranean population of cultured meagre, Argyrosomus regius Asso (Perciformes:Sciaenidae) / J. Fish Dis 36 : 141 ~ 149
Huang YC / 2008 / Isolation and identification of the pathogen causing sarcoidosis of Trachinotus ovatus / J. Guangdong Ocean Univ 28 : 49 ~ 53
Cai SH / 2010 / Loop-mediated isothermal amplification method for rapid detection of Vibrio alginolyticus, the causative agent of vibriosis in mariculture fish / Lett. Appl.
Chen SC / 2000 / Nocardiosis in sea bass, Lateolabrax japonicus, in Taiwan / J. Fish Dis 23 : 299 ~ 307
Elkesh A / 2013 / Systemic nocardiosis in a Mediterranean population of cultured meagre, Argyrosomus regius Asso (Perciformes:Sciaenidae) / J. Fish Dis 36 : 141 ~ 149
Huang YC / 2008 / Isolation and identification of the pathogen causing sarcoidosis of Trachinotus ovatus / J. Guangdong Ocean Univ 28 : 49 ~ 53
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