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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제4호
- 발행연도
- 2015.1
- 수록면
- 511 - 520 (10page)
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Triclosan, the widely used biocide, specifically targets enoyl-acyl carrier protein reductase (ENR) in the bacterial fatty acid synthesis system. Although the fish pathogen Aeromonas salmonicida subsp. salmonicida exhibits triclosan resistance, the nature of this resistance has not been elucidated. Here, we aimed to characterize the triclosan resistance of A. salmonicida subsp. salmonicida causing furunculosis. The fosmid library of triclosan-resistant A. salmonicida subsp. salmonicida was constructed to select a fosmid clone showing triclosan resistance. With the fosmid clone showing triclosan resistance, a subsequent secondary library search resulted in the selection of subclone pTSR-1. DNA sequence analysis of pTSR-1 revealed the presence of a chromosomal-borne fabV-encoding ENR homolog. The ENR of A. salmonicida (FabVas) exhibited significant homology with previously known FabV, including the catalytic domain YX(8)K. fabVas introduction into E. coli dramatically increased its resistance to triclosan. Heterologous expression of FabVas might functionally replace the triclosan-sensitive FabI in vivo to confer E. coli with triclosan resistance. A genome-wide search for fabVas homologs revealed the presence of an additional fabV gene (fabVas2) paralog in A. salmonicida strains and the fabVas orthologs from other gram-negative fish pathogens. Both of the potential FabV ENRs expressed similarly with or without triclosan supplement. This is the first report about the presence of two potential FabV ENRs in a single pathogenic bacterium. Our result suggests that triclosan-resistant ENRs are widely distributed in various bacteria in nature, and the wide use of this biocide can spread these triclosan-tolerant ENRs among fish pathogens and other pathogenic bacteria.
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참고문헌 (25)
참고문헌 신청
Amos GC / / Functional metagenomic analysis reveals rivers are a reservoir for diverse antibiotic resistance genes. Vet. Microbiol
Bailey AM / 2008 / RamA confers multidrug resistance in Salmonella enterica via increased expression of acrB, which is inhibited by chlorpromazine / Antimicrob. Agents Chemot
Bergler H / 1992 / Sequences of the envM gene and of two mutated alleles in Escherichia coli / J. Gen. Microbiol 138 : 2093 ~ 2100
Bergler H / 1994 / Protein EnvM is the NADHdependent enoyl-ACP reductase (FabI) of Escherichia coli / J. Biol. Chem 269 : 5493 ~ 5496
Byrne-Bailey KG / 2011 / Integron prevalence and diversity in manured soil / Appl. Environ. Microbiol 77 : 684 ~ 687
Bailey AM / 2008 / RamA confers multidrug resistance in Salmonella enterica via increased expression of acrB, which is inhibited by chlorpromazine / Antimicrob. Agents Chemot
Bergler H / 1992 / Sequences of the envM gene and of two mutated alleles in Escherichia coli / J. Gen. Microbiol 138 : 2093 ~ 2100
Bergler H / 1994 / Protein EnvM is the NADHdependent enoyl-ACP reductase (FabI) of Escherichia coli / J. Biol. Chem 269 : 5493 ~ 5496
Byrne-Bailey KG / 2011 / Integron prevalence and diversity in manured soil / Appl. Environ. Microbiol 77 : 684 ~ 687
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