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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제4호
- 발행연도
- 2015.1
- 수록면
- 547 - 553 (7page)
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The yeast surface-displayed cDNA library has been used to identify unknown antigens. However, when unknown target antigens show moderate-to-low abundance, some modifications are needed in the screening process. In this study, a directional random-primed cDNA library was used to increase the number of candidates for the unknown antigen. To avoid the loss of target yeast clones that express proteins at a low frequency in the cDNA library, a comprehensive monitoring system based on magnetic-activated cell sorting, fluorescence-activated cell sorting, and immunofluorescence was established, and a small number of target yeast cells was successfully enriched. These results showed that our optimized method has potential application for identifying rare unknown antigens of the human monoclonal antibody.
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참고문헌 (20)
참고문헌 신청
Bidlingmaier S / 2006 / Construction and application of a yeast surface-displayed human cDNA library to identify post-translational modification-dependent protein-protein int
Bidlingmaier S / 2009 / Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelio
Bidlingmaier S / 2011 / Construction of yeast surfacedisplayed cDNA libraries / Methods Mol. Biol 729 : 199 ~ 210
Chao G / 2006 / Isolating and engineering human antibodies using yeast surface display / Nat. Protoc 1 : 755 ~ 768
Daugherty PS / 2000 / Flow cytometric screening of cell-based libraries / J. Immunol. Methods 243 : 211 ~ 227
Bidlingmaier S / 2009 / Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelio
Bidlingmaier S / 2011 / Construction of yeast surfacedisplayed cDNA libraries / Methods Mol. Biol 729 : 199 ~ 210
Chao G / 2006 / Isolating and engineering human antibodies using yeast surface display / Nat. Protoc 1 : 755 ~ 768
Daugherty PS / 2000 / Flow cytometric screening of cell-based libraries / J. Immunol. Methods 243 : 211 ~ 227
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