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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제8호
- 발행연도
- 2015.1
- 수록면
- 1,324 - 1,327 (4page)
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초록· 키워드
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The nucleotide sequence of the TRP1 gene encoding phosphoribosyl anthranilate isomerase in yeast Saccharomycopsis fibuligera was determined by degenerate polymerase chain reaction and genome walking. Sequence analysis revealed the presence of an uninterrupted open-reading frame of 759 bp, including the stop codon, encoding a 252 amino acid residue. The deduced amino acid sequence of Trp1 in S. fibuligera was 43.5% homologous to that of Komagataella pastoris. The cloned TRP1 gene (SfTRP1) complemented the trp1 mutation in Saccharomyces cerevisiae, suggesting that it encodes a functional TRP1 in S. fibuligera. A new auxotrophic marker to engineer starch-degrading yeast S. fibuligera is now available. The GenBank Accession No. for SfTRP1 is KR078268.
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등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Bjellqvist B / 1993 / The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences / Electrophoresis 14 : 1023 ~ 1031
Braus GH / 1991 / Aromatic amino acid biosynthesis in the yeast Saccharomyces cerevisiae: a model system for the regulation of a eukaryotic biosynthetic pathway / Microbiol.
Cheon SA / 2003 / Isolation and characterization of the TRP1 gene from the yeast Yarrowia lipolytica and multiple gene disruption using a TRP blaster / Yeast 20 : 677 ~ 685
Chi Z / 2009 / Saccharomycopsis fibuligera and its applications in biotechnology / Biotechnol. Adv 27 : 423 ~ 431
Cosano I / 1998 / Cloning and sequence analysis of the Pichia pastoris TRP1, IPP1 and HIS3 genes / Yeast 14 : 861 ~ 867
Braus GH / 1991 / Aromatic amino acid biosynthesis in the yeast Saccharomyces cerevisiae: a model system for the regulation of a eukaryotic biosynthetic pathway / Microbiol.
Cheon SA / 2003 / Isolation and characterization of the TRP1 gene from the yeast Yarrowia lipolytica and multiple gene disruption using a TRP blaster / Yeast 20 : 677 ~ 685
Chi Z / 2009 / Saccharomycopsis fibuligera and its applications in biotechnology / Biotechnol. Adv 27 : 423 ~ 431
Cosano I / 1998 / Cloning and sequence analysis of the Pichia pastoris TRP1, IPP1 and HIS3 genes / Yeast 14 : 861 ~ 867
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