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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제2호
- 발행연도
- 2016.1
- 수록면
- 226 - 232 (7page)
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Dihydrodipicolinate reductase is an enzyme that converts dihydrodipicolinate to tetrahydrodipicolinate using an NAD(P)H cofactor in L-lysine biosynthesis. To increase the understanding of the molecular mechanisms of lysine biosynthesis, we determined the crystal structure of dihydrodipicolinate reductase from Corynebacterium glutamicum (CgDapB). CgDapB functions as a tetramer, and each protomer is composed of two domains, an Nterminal domain and a C-terminal domain. The N-terminal domain mainly contributes to nucleotide binding, whereas the C-terminal domain is involved in substrate binding. We elucidated the mode of cofactor binding to CgDapB by determining the crystal structure of the enzyme in complex with NADP+ and found that CgDapB utilizes both NADH and NADPH as cofactors. Moreover, we determined the substrate binding mode of the enzyme based on the coordination mode of two sulfate ions in our structure. Compared with Mycobacterium tuberculosis DapB in complex with its cofactor and inhibitor, we propose that the domain movement for active site constitution occurs when both cofactor and substrate bind to the enzyme.
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참고문헌 (20)
참고문헌 신청
Brünger AT / 1998 / Crystallography & NMR system: a new software suite for macromolecular structure determination / Acta Crystallogr. D Biol. Crystallogr 54:905~921
Cirilli M / 2003 / The threedimensional structures of the Mycobacterium tuberculosis dihydrodipicolinate reductase-NADH-2,6-PDC and-NADPH-2,6-PDC complexes. Structural and mutagenic analysis of relaxed nucleotide specificity / Biochemistry 42:10644~10650
Emsley P / 2004 / Coot: model-building tools for molecular graphics / Acta Crystallogr. D Biol. Crystallogr 60:2126~2132
Ikeda M / 2003 / The Corynebacterium glutamicum genome: features and impacts on biotechnological processes / Appl. Microbiol. Biotechnol 62:99~109
Ikeda M / 2013 / Corynebacterium glutamicum / Springer :107~147
Cirilli M / 2003 / The threedimensional structures of the Mycobacterium tuberculosis dihydrodipicolinate reductase-NADH-2,6-PDC and-NADPH-2,6-PDC complexes. Structural and mutagenic analysis of relaxed nucleotide specificity / Biochemistry 42:10644~10650
Emsley P / 2004 / Coot: model-building tools for molecular graphics / Acta Crystallogr. D Biol. Crystallogr 60:2126~2132
Ikeda M / 2003 / The Corynebacterium glutamicum genome: features and impacts on biotechnological processes / Appl. Microbiol. Biotechnol 62:99~109
Ikeda M / 2013 / Corynebacterium glutamicum / Springer :107~147
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