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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제2호
- 발행연도
- 2015.1
- 수록면
- 288 - 295 (8page)
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초록· 키워드
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Salmonella enterica serovar Enteritidis is the predominant agent causing salmonellosis in chickens and other domestic animals. In an attempt to identify antigenic S. Enteritidis outer membrane proteins (OMPs) that may be useful for subunit vaccine development, we established a proteomic map and database of antigenic S. Enteritidis OMPs. In total, 351 and 301 spots respectively from S. Enteritidis strain 270 and strain 350 were detected by twodimensional gel electrophoresis. Fifty-one antigen-reactive spots were detected by antisera on two-dimensional immunoblots and identified as 12 specific proteins by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. OmpA and DNA starvation/ stationary phase protection protein (Dps) were the most abundant proteins among the identified OMPs, comprising 22 and 12 protein species, respectively. Interestingly, we found that the Dps of S. Enteritidis is also antigenic. OmpW was also verified to have high antigenicity. These results show that OmpA, Dps, and possibly OmpW are antigenic proteins. This study provides new insights into our understanding of the immunogenic characteristics of S. Enteritidis OMPs.
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참고문헌 (23)
참고문헌 신청
Alam SI / 2009 / Differential proteomic analysis of Clostridium perfringens ATCC13124; identification of dominant, surface and structure associated proteins / BMC Microbiol 9
Barenkamp SJ / 1981 / Subtyping isolates of Haemophilus influenzae type b by outer-membrane protein profiles / J. Infect. Dis 143 : 668 ~ 676
Baumler AJ / 2000 / Tracing the origins of Salmonella outbreaks / Science 287 : 50 ~ 52
Belaaouaj A / 2000 / Degradation of outer membrane protein A in Escherichia coli killing by neutrophil elastase / Science 289 : 1185 ~ 1188
Bini L / 1996 / Mapping of Chlamydia trachomatis proteins by immobiline-polyacrylamide two-dimensional electrophoresis: spot identification by N-terminal sequencing and immun
Barenkamp SJ / 1981 / Subtyping isolates of Haemophilus influenzae type b by outer-membrane protein profiles / J. Infect. Dis 143 : 668 ~ 676
Baumler AJ / 2000 / Tracing the origins of Salmonella outbreaks / Science 287 : 50 ~ 52
Belaaouaj A / 2000 / Degradation of outer membrane protein A in Escherichia coli killing by neutrophil elastase / Science 289 : 1185 ~ 1188
Bini L / 1996 / Mapping of Chlamydia trachomatis proteins by immobiline-polyacrylamide two-dimensional electrophoresis: spot identification by N-terminal sequencing and immun
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