지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.1
- 수록면
- 636 - 642 (7page)
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초록· 키워드
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Background: CpG DNA plays an important role in immune cell function. This study examined whether the temporal control of toll-like receptor (TLR)9 by CpG DNA can regulate the expression of matrix metalloproteinase-9 (MMP-9).
Methods and materials: Macrophages were cultured in the presence of 10% FBS. For the various MMP genes analysis, RT-PCR and real-time PCR were performed. In addition, zymography assay performed for the MMP activity. The phosphorylation assay did for the ERK1/2 and NFκB activation, and luciferase promoter assay was for the NFκB activity.
Results: CpG DNA induced the mRNA expression of MMP-2, MMP-9, and MMP-13, but not of MMP-7, MMP-8, and MMP-12, in a time-dependent manner. Especially, the mRNA expression of MMP-9 was strongly induced by CpG DNA using real-time RT-PCR. The TLR9 inhibitor, chloroquine, suppressed CpG DNA-induced MMP-9 expression and its activity. Moreover, CpG DNA induced the phosphorylation of ERK and the inhibition of ERK by U0126 suppressed CpG DNA-induced MMP-9 expression and its activity. CpG DNA stimulated IκB-αdegradation and luciferase activity. In addition, pretreatment of SN-50, the inhibitor of NFκB, strongly blocked the CpG DNA-induced MMP-9 expression and activity.
Conclusion: These observations suggest that CpG DNA may play important roles in the activation of macrophages by regulating the production of MMP-9 via the sequential TLR9-ERK-NFκB signaling pathway.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
/ 1998 / CpG DNA rescue from anti-IgM-induced WEHI-231 B lymphoma apoptosis via modulation of I kappa B alpha and I kappa B beta and sustained activation of nuclear factor-k
/ 2000 / Immune cell activation by bacterial CpG-DNA through myeloid differentiation marker 88 and tumor necrosis factor receptor-associated factor (TRAF)6 192 : 595 ~ 600
/ 2000 / Toll signaling pathways in the innate immune response 12 : 13 ~ 19
/ 2002 / CpG motifs in bacterial DNA and their immune effects 20 : 709 ~ 760
/ 2002 / Interferons inhibit tumor necrosis factor-alpha-mediated matrix metalloproteinase-9 activation via interferon regulatory factor-1 binding competition with NF-kappa
/ 2000 / Immune cell activation by bacterial CpG-DNA through myeloid differentiation marker 88 and tumor necrosis factor receptor-associated factor (TRAF)6 192 : 595 ~ 600
/ 2000 / Toll signaling pathways in the innate immune response 12 : 13 ~ 19
/ 2002 / CpG motifs in bacterial DNA and their immune effects 20 : 709 ~ 760
/ 2002 / Interferons inhibit tumor necrosis factor-alpha-mediated matrix metalloproteinase-9 activation via interferon regulatory factor-1 binding competition with NF-kappa
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