지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 489 - 497 (9page)
이용수
초록· 키워드
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ObjectiveThe aim of this study was to describe the principle of the Cheil HPV DNA Chip assay and evaluate its accuracy. In order to quantify the human papillomavirus (HPV) load and identify HPV genotypes simultaneously, this assay combined the two methods: SYBR Green quantitative real-time polymerase chain reaction (PCR) and DNA microarray.
MethodsWe designed novel consensus primer sets that target the conserved region of the HPV L1 gene for quantifying and detecting a broad range of HPV types by quantitative real-time PCR. Subsequently, using the PCR products, DNA microarray was performed with 36 HPV type-specific probes. To validate this method, direct sequencing and correlation analysis among HPV genotype, viral load, and cytological abnormality was performed by Cohen’s kappa values, two-sided McNemar chi-square test, Kruskal-Wallis test, and odds ratios.
ResultsThe kappa value of the Cheil HPV DNA Chip was 0.963 (95% confidence interval, 0.919 to 0.98), which was significantly higher than the value of 0.527 (95% confidence interval, 0.447 to 0.59) obtained using a conventional HPV DNA Chip. HPV16 (χ2=62.28, P<0.01), HPV33 (χ2=7.18, P<0.01), and HPV58 (χ2=9.52, P<0.01), which are classified as high-risk HPVs, were detected at significant levels in samples with high-grade lesions. And viral loads tended to be higher in groups with high odds ratios.
ConclusionThe Cheil HPV DNA Chip is an effective diagnostic assay for simultaneously detecting HPV genotypes and loads in cervical samples.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Dalstein V / 2003 / Persistence and load of highrisk HPV are predictors for development of high-grade cervical lesions : a longitudinal French cohort study / Int J Cancer 106:396~403
Schlecht NF / 2001 / Persistent human papillomavirus infection as a predictor of cervical intraepithelial neoplasia / JAMA 286:3106~3114
Lorincz AT / 1992 / Human papillomavirus infection of the cervix : relative risk associations of 15 common anogenital types / Obstet Gynecol 79:328~337
Walboomers JM / 1999 / Human papillomavirus is a necessary cause of invasive cervical cancer worldwide / J Pathol 189:12~19
Heard I / 2000 / Increased risk of cervical disease among human immunodeficiency virus-infected women with severe immunosuppression and high human papillomavirus load / Obstet Gynecol 96:403~409
Schlecht NF / 2001 / Persistent human papillomavirus infection as a predictor of cervical intraepithelial neoplasia / JAMA 286:3106~3114
Lorincz AT / 1992 / Human papillomavirus infection of the cervix : relative risk associations of 15 common anogenital types / Obstet Gynecol 79:328~337
Walboomers JM / 1999 / Human papillomavirus is a necessary cause of invasive cervical cancer worldwide / J Pathol 189:12~19
Heard I / 2000 / Increased risk of cervical disease among human immunodeficiency virus-infected women with severe immunosuppression and high human papillomavirus load / Obstet Gynecol 96:403~409
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