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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 1 - 7 (7page)
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To meet the increasing demands of specific pathogen free (SPF) minipigs in biomedical researches, 8 pregnantChinese Wuzhishan minipigs (WZSP) sows with clear background were chosen to obtain SPF WZSP byhysterectomy. At 111 ± 2 days of the pregnancy, piglets were aseptically taken out from the sows and artificiallysuckled for 40 to 45 days in the positive isolators. Then, the piglets defined as F0 were transferred to barrierenvironment and fed with standard feeds. The original SPF colony was formed for breeding by selected pigletsfrom F0 group of 6–8 months old. Biological characteristics of SPF WZSP were collected and further compared tothose of conventional (CV) WZSP, including growth performance, reproductive performance, hematology and bloodbiochemistry, and major pathogens detection. As a result, 61 F0 piglets were obtained from 8 candidate sows, and55 out of them survived. After strictly selection, 35 F0 piglets were used to form the original SPF colony, whichproduced 14 litters of SPF piglets defined as F1. Piglet survival rates, growth performance, and reproductiveperformance of SPF WZSP were similar to CV WZSP. Some hematology and blood biochemistry parameters showedsignificant differences between SPF and CV WZSP. Eighteen kinds of pathogens were identified to be free in F0 andF1 SPF colony by repeated pathogen detections. In conclusion, we established a satisfied SPF WZSP colonymaintaining original characteristics, free of controlled diseases, and being proved to be a suitable laboratory animal
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등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Carroll JA / 2000 / Postnatal function of the somatotrophic axis in pigs born naturally or by Caesarian section / Domest Anim Endocrinol 19 : 39 ~ 52
Dong X / 2014 / Generation of chimeric piglets by injection of embryonic germ cells from inbred Wuzhishan miniature pigs into blastocysts / Xenotransplantation 21 (2) : 140 ~
Havill LM / 2003 / Hematology and blood biochemistry in infant baboons(Papio hamadryas) / J Med Primato 32 (3) : 131 ~ 138
Herndon JG / 2001 / Hematologic and blood biochemical variables of captive chimpanzees : cross-sectional and longitudinal analyses / Comp Med 51 (1) : 60 ~ 69
Johnson P / / Dogs in Biomedical Research
Dong X / 2014 / Generation of chimeric piglets by injection of embryonic germ cells from inbred Wuzhishan miniature pigs into blastocysts / Xenotransplantation 21 (2) : 140 ~
Havill LM / 2003 / Hematology and blood biochemistry in infant baboons(Papio hamadryas) / J Med Primato 32 (3) : 131 ~ 138
Herndon JG / 2001 / Hematologic and blood biochemical variables of captive chimpanzees : cross-sectional and longitudinal analyses / Comp Med 51 (1) : 60 ~ 69
Johnson P / / Dogs in Biomedical Research
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