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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Background: Malassezia yeasts as major pathogenic fungi causes the common skin diseases including dandruff, psoriasis, seborrheic dermatitis and atopic dermatitis etc. various molecular techniques were developed to identify and classify the Malassezia species until now. But, these methods were discovered the problems. So, the development of the better molecular methods required to identify and classify of Malasseiza species.
Objective: We sought to develop of molecular techniques to identify and classify of six Malassezia species (M. restricta, M. globosa, M. furfur, M. slooffiae, M. sympodialis, M. obtusa).
Methods: We designed primers about ITS1 (Internal transcribed space 1) region that were wellknown region useful to identify of Malassezia species. Because, ITS1 region that is located between 18S and 5.8S rDNA of ribosomal DNA was comparatively mutated quickly. The mono PCR using ITS1 primers was performed to confirm the specificity of ITS1 primers with six Malassezia standard strains. Then, Malassezia Multiplex detection kit was developed on the basis of technique using ITS1regions. Malassezia Multiplex detection kit was used to perform multiplex PCR with six Malassezia standard strains and clinical isolates.
Results: The results of mono PCR using ITS1 primers about six Malassezia standard strains was detected each Malassezia standard strains. Also, the multiplex PCR using developed Malassezia Multiplex detection kit was confirmed to classify about six Malassezia standard strains and clinical isolates.
Conclusion: In this study, we verified that six Malassezia yeasts was classified using Malassezia Multiplex detection kit from Malasszia standard strains and clinical isolates. And we anticipate that Malassezia Multiplex detection kit is able to do accurate diagnosis about six Malassezia yeasts (M.restricta, M. globosa, M. furfur, M. slooffiae, M. sympodialis, M. obtusa).
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참고문헌 (15)
참고문헌 신청
AJ Kindo / 2004 / Identification of Malassezia species / Indian Journal of Medical Microbiology 22 (3) : 179 ~ 181
Aditya K / 2000 / Molecular differentiation of seven Malassezia species / J Clin Microbiol 38 (5) : 1869 ~ 1875
Bart T / 2001 / Identification and typing of Malassezia yeasts using amplified fragment length polymorphism (AFLPTM), random amplified polymorphic DNA (RAPD) and denaturing
Christina M / 2002 / Fast, noninvasive method for molecular detection and differentiation of Malassezia yeast species on human skin and application of the method to Dandruff
Gaitanis G / 2002 / Identification of Malassezia species from patient skin scales by PCRRFLP / Clin Microbiol Infect 8 : 162 ~ 173
Aditya K / 2000 / Molecular differentiation of seven Malassezia species / J Clin Microbiol 38 (5) : 1869 ~ 1875
Bart T / 2001 / Identification and typing of Malassezia yeasts using amplified fragment length polymorphism (AFLPTM), random amplified polymorphic DNA (RAPD) and denaturing
Christina M / 2002 / Fast, noninvasive method for molecular detection and differentiation of Malassezia yeast species on human skin and application of the method to Dandruff
Gaitanis G / 2002 / Identification of Malassezia species from patient skin scales by PCRRFLP / Clin Microbiol Infect 8 : 162 ~ 173
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