지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.10
- 수록면
- 915 - 931 (17page)
- DOI
- 10.35159/kjss.2019.10.28.5.915
이용수
초록· 키워드
오류제보하기
PURPOSE: This study was to investigate the functional role of ryanodine receptor(RYR) on palmitateinduced metabolic changes which related with endoplasmic/sarcoplasmic reticulum stress(ER) and mitochondria function.
METHODS: To examine the function of RYR on palmitate-induced ER stress and mitochondria dysfunction, 100uM palmitate was treated with C2C12 cell. Palmitate-induced reactive oxygen species (ROS) formation was measured by DCFDA fluorescence dye. ER Ca<SUP>2+</SUP> level was measured by using thapsigargin-induced ER Ca<SUP>2+</SUP> release method which was detected by Flou-4 am, Ca<SUP>2+</SUP> dye. palmitateinduced mitochondria potential was measured by TMRM dye. ER stress protein IRE1-alpha expression, phosphorylation, and mitochondria byosynthesis related protein, PGC1 alpha, ACC and UCP2 were analyzed by western blotting. All parameters were compared between control cell, palmitate - treated cell and palmitate and dantrolene - treated cell.
RESULTS: Palmitate significantly increases intracellular ROS and ER stress marker and also increases ER Ca<SUP>2+</SUP> depletion. Palmitate decreases mitochondrial potential and mitochondria biosynthesis related protein expression and phosphorylation. Beside of these results, dantrolene treated C2C12 cells significantly ameliorated palmitate-induced ER Ca<SUP>2+</SUP>depletion, ER stress marker increase and mitochondrial potential decrease compared with control cell. These results suggest RYR channel was involved with palmitate-induced ER depletion, mitochondria dysfunction and ER Ca<SUP>2+</SUP> level coincides with mitochondria potential and function.
CONCLUSIONS: These results suggest that palmitate increase ER Ca2+ depletion and decrease mitochondria function. RYR channel could involve palmitate-induced ER Ca2+ depletion process and plays a pivotal role in palmitate-induced the progression of ER stress and mitochondria dysfunction.
METHODS: To examine the function of RYR on palmitate-induced ER stress and mitochondria dysfunction, 100uM palmitate was treated with C2C12 cell. Palmitate-induced reactive oxygen species (ROS) formation was measured by DCFDA fluorescence dye. ER Ca<SUP>2+</SUP> level was measured by using thapsigargin-induced ER Ca<SUP>2+</SUP> release method which was detected by Flou-4 am, Ca<SUP>2+</SUP> dye. palmitateinduced mitochondria potential was measured by TMRM dye. ER stress protein IRE1-alpha expression, phosphorylation, and mitochondria byosynthesis related protein, PGC1 alpha, ACC and UCP2 were analyzed by western blotting. All parameters were compared between control cell, palmitate - treated cell and palmitate and dantrolene - treated cell.
RESULTS: Palmitate significantly increases intracellular ROS and ER stress marker and also increases ER Ca<SUP>2+</SUP> depletion. Palmitate decreases mitochondrial potential and mitochondria biosynthesis related protein expression and phosphorylation. Beside of these results, dantrolene treated C2C12 cells significantly ameliorated palmitate-induced ER Ca<SUP>2+</SUP>depletion, ER stress marker increase and mitochondrial potential decrease compared with control cell. These results suggest RYR channel was involved with palmitate-induced ER depletion, mitochondria dysfunction and ER Ca<SUP>2+</SUP> level coincides with mitochondria potential and function.
CONCLUSIONS: These results suggest that palmitate increase ER Ca2+ depletion and decrease mitochondria function. RYR channel could involve palmitate-induced ER Ca2+ depletion process and plays a pivotal role in palmitate-induced the progression of ER stress and mitochondria dysfunction.
목차
Abstract
Ⅰ. 서론
Ⅱ. 연구방법
Ⅲ. 결과
Ⅳ. 논의
Ⅴ. 결론
참고문헌
참고문헌 (47)
참고문헌 신청
Aas, V. / 2005 / Lipid metabolism in human skeletal muscle cells : effects of palmitate and chronic hyperglycaemia / Acta Physiol Scand 183(1):31~41
Akerfeldt, M. C. / 2008 / Cytokine-induced β-cell death is independent of endoplasmic reticulum stress signaling / Diabetes 57:3034~3044
Arruda, A. P. / 2014 / Chronic enrichment of hepatic endoplasmic reticulum-mitochondria contact leads to mitochondrial dysfunction in obesity / Nat Med 20:1427~1435
Boudina, S. / 2007 / Mitochondrial energetics in the heart in obesity-related diabetes : direct evidence for increased uncoupled respiration and activation of uncoupling proteins / Diabetes 56:2457~2466
Chavez, J. A. / 2003 / Characterizing the effects of saturated fatty acids on insulin signaling and ceramide and diacylglycerol accumulation in 3T3-L1 adipocytes and C2C12 myotubes / Arch Biochem Biophys 419:101~109
Akerfeldt, M. C. / 2008 / Cytokine-induced β-cell death is independent of endoplasmic reticulum stress signaling / Diabetes 57:3034~3044
Arruda, A. P. / 2014 / Chronic enrichment of hepatic endoplasmic reticulum-mitochondria contact leads to mitochondrial dysfunction in obesity / Nat Med 20:1427~1435
Boudina, S. / 2007 / Mitochondrial energetics in the heart in obesity-related diabetes : direct evidence for increased uncoupled respiration and activation of uncoupling proteins / Diabetes 56:2457~2466
Chavez, J. A. / 2003 / Characterizing the effects of saturated fatty acids on insulin signaling and ceramide and diacylglycerol accumulation in 3T3-L1 adipocytes and C2C12 myotubes / Arch Biochem Biophys 419:101~109
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
혈관기능 항상성을 위한 유산소성 운동과 미토콘드리아
운동과학
2018 .01
-
근수축시 해당작용에 의한 근형질 세망의 Ca2+ 변화가 미토콘드리아 Ca2+ 증가에 미치는 영향
운동과학
2017 .08
-
유산소성 운동 강도와 금식 시간이 쥐의 골격근내 AMPK, PGC-1α, PPARα및 GLUT4 단백질 발현에 미치는 영향
한국체육과학회지
2016 .02
-
트레드밀 지구성 운동이 알츠하이머 형질전환 생쥐 뇌의 mitochondrial dynamic과 신경세포생존에 미치는 영향
운동과학
2016 .01
-
Prolonged Hypoxic Exposure Impairs Endothelial Functions: Possible Mechanism of HIF-1α Signaling
운동과학
2024 .05
-
근수축에 의한 Nitric Oxide 생성과 미토콘드리아 생합성 조절체계에서 CaMKII의 작용
운동과학
2016 .01
-
The Cell
한국기초조형학회 작품집
2023 .12
-
트레드밀 운동이 고지방 식이 쥐의 해마에서의 소포체 스트레스와 인슐린 저항성에 미치는 영향
운동과학
2015 .01
-
엘리트 장애인 선수들의 스트레스 요인 탐색연구
한국체육과학회지
2016 .04
-
응급실 폭력예방을 위한 서비스디자인 -고려대병원 응급실 사례 중심으로-
기초조형학연구
2016 .06
-
발기 부전에서 유산소성 운동의 역할: 무작위 통제 연구의 고찰
운동과학
2021 .01
-
Resistance Exercise Training inhibit Endoplasmic Reticulum Stress in the Aorta of Zucker Diabetic Fatty Rats
한국체육과학회지
2018 .12
-
Stress Management Design Guideline with Smart Devices during COVID-19
Archives of Design Research
2022 .11
-
Mothering - 9th year
International Invitation Exhibition of Color Works
2017 .11
-
노인 남녀의 일상생활체력 측정을 이용한 VO2max 추정식 제안
한국발육발달학회지
2020 .05
-
[New Album] 작곡가 정애련 여섯번째 작품집 단향緞香
월간 리뷰
2021 .02
-
[Music Space] 이제는 공간에 투자해야 할 때 공간 음향의 새로운 대안, 메이어사운드 - 컨스털레이션 Constellation
월간 리뷰
2021 .04
-
반복적인 근 수축으로 인한 ROS 방어 체계의 향상이 Palmitate로 인한 ER stress 유발 및 미토콘드리아 칼슘에 미치는 효과
한국체육과학회지
2020 .02
-
New insights for anti-aging activity of the active molecules that have been encapsulated in living plant cells
한국화장품미용학회지
2015 .06
-
[PORTFOLIO] 자연의 변주變奏
월간사진
2022 .05
이 논문의 저자 정보
이 논문과 함께 이용한 논문
반복적인 근 수축으로 인한 ROS 방어 체계의 향상이 Palmitate로 인한 ER stress 유발 및 미토콘드리아 칼슘에 미치는 효과
한진섭
,
김상현
,
박대령
한국체육과학회지
2020
.02
미토콘드리아 기능 이상과 암
한유선
,
제갈명은
,
김영진
생명과학회지
2019
.09
암줄기세포의 특성 및 면역관문억제
최상훈
,
김형기
생명과학회지
2019
.04
암 진단 분자 마커로서 이동성 유전인자의 응용
김혜민
,
김정안
,
우효정
외 3명
생명과학회지
2017
.10
지구성 훈련에 반응한 골격근의 미토콘드리아 항상성 조절
주정선
생명과학회지
2017
.03
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2019-692-001319318