지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
Sirtuins are NAD+-dependent deacetylase that are broadly conserved throughout bacteria, archaea, and eukaryotes. Themembers of sirtuins are important in regulating diverse biological pathways, including gene silencing, DNA repair, genomestability, longevity, metabolism, and cell physiology. Sirtuin from Bacillus amyloliquefaciens (BaSrtN) is a particularlyinteresting bacterial Sir2 homologue. In this study, to further understand the function and mechanisms of this protein,BaSrtN was successfully expressed and purified using Ni-NTA affinity, Q anion-exchange, and gel-filtration chromatography. Purified BaSrtN was crystallized and diffracted to the resolution of 1.45 Å. The preliminary crystallographic analysissuggested that BaSrtN crystal belongs to the trigonal space group P31 or P32, with unit-cell parameters of a = b = 90.115and c = 86.306 Å. Size-exclusion chromatography suggested that BaSrtN prefer to exit as monomers in solution.
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등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Avalos, J. L. / 2005 / Mechanism of sirtuin inhibition by nicotinamide : altering the NAD(+)cosubstrate specificity of a Sir2 enzyme / Mol Cell 17:855~868
Balan, V. / 2008 / Life span extension and neuronal cell protection by Drosophila nicotinamidase / J Biol Chem 283:27810~27819
Braunstein, M. / 1993 / Transcriptional silencing in yeast is associated with reduced nucleosome acetylation / Genes Dev 7:592~604
Bryk, M. / 2002 / Evidence that Set1, a factor required for methylation of histone H3, regulates rDNA silencing in S. cerevisiae by a Sir2-independent mechanism / Curr Biol 12:165~170
Dang, W. / 2014 / The controversial world of sirtuins / Drug Discov Today Technol 12:e9~e17
Balan, V. / 2008 / Life span extension and neuronal cell protection by Drosophila nicotinamidase / J Biol Chem 283:27810~27819
Braunstein, M. / 1993 / Transcriptional silencing in yeast is associated with reduced nucleosome acetylation / Genes Dev 7:592~604
Bryk, M. / 2002 / Evidence that Set1, a factor required for methylation of histone H3, regulates rDNA silencing in S. cerevisiae by a Sir2-independent mechanism / Curr Biol 12:165~170
Dang, W. / 2014 / The controversial world of sirtuins / Drug Discov Today Technol 12:e9~e17
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