지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
The emergences of multi-drug resistant bacteria such as Acinetobacter baumanni have emphasized the necessity ofnew antibiotics. Peptidyl deformylase (PDF) catalyzes the removal of the formyl group from the N-terminal formylatedmethionine residue present in all nascent polypeptides in bacteria. In this study, the PDF gene from Acinetobacterbaumannii K0420859 was cloned and its protein was overexpressed in E. coli, purified, and crystallized. The purifiedprotein was crystallized using the hanging-drop vapour-diffusion method and the crystal diffracted to 2.4 A resolution. Thecrystal belonged to the trigonal space group P32 with unit cell parameters of a = b= 39.4 A and c = 187.9 A. Two protomerswere presented in the asymmetric unit with a corresponding VM of 2.10 A3 Da-1 and a solvent content of 41.5%.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Antunes, L.C.S / 2011 / Deciphering the multifactorial nature of Acinetobacter baumannii pathogenicity / PLoS One 6:e22674
Axten, J. M / 2012 / Acylprolinamides: a new class of peptide deformylase inhibitors with in vivo antibacterial activity / Bioorg Med Chem Lett 22:4028~4032
Chang, S. Y / 1989 / Methionine aminopeptidase gene of Escherichia coli is essential for cell growth / J Bacteriol 171:4071~4072
Chen, D. Z / 2000 / Actinonin, a naturally occurring antibacterial agent, is a potent deformylase inhibitor / Biochemistry 39:1256~1262
Clements, J. M / 2001 / Antibiotic activity and characterization of BB-3497, a novel peptide deformylase inhibitor / Antimicrob Agents Chemother 45:563~570
Axten, J. M / 2012 / Acylprolinamides: a new class of peptide deformylase inhibitors with in vivo antibacterial activity / Bioorg Med Chem Lett 22:4028~4032
Chang, S. Y / 1989 / Methionine aminopeptidase gene of Escherichia coli is essential for cell growth / J Bacteriol 171:4071~4072
Chen, D. Z / 2000 / Actinonin, a naturally occurring antibacterial agent, is a potent deformylase inhibitor / Biochemistry 39:1256~1262
Clements, J. M / 2001 / Antibiotic activity and characterization of BB-3497, a novel peptide deformylase inhibitor / Antimicrob Agents Chemother 45:563~570
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Purification, crystallization and preliminary X-ray crystallographic analysis of VCA0593 with a c-di-GMP binding activity in Vibrio cholerae
Biodesign
2017 .06
-
Purification, crystallization and X-ray crystallographic analysis of cystathionine gamma-synthase from Corynebacterium glutamicum
Biodesign
2017 .03
-
Crystallization and preliminary X-ray diffraction analysis of a toxin-antitoxin MazEF complex from the extremophile Deinococcus radiodurans
Biodesign
2018 .03
-
Purification, Crystallization and X-ray crystallographic analysis of Homoserine O-succinyltransferase from Escherichia coli
Biodesign
2019 .01
-
Purification, crystallization and X-ray crystallographic analysis of glycine oxidase from Bacillus cereus ATCC 14579
Biodesign
2020 .01
-
Preliminary crystallographic study of CD38 in complex with isatuximab, an FDA-approved antibody drug for treating multiple myeloma
Biodesign
2020 .01
-
Purification, crystallization, and X-ray crystallographic analysis of fatty acid amide hydrolase from Candida albicans
Biodesign
2019 .01
-
Differential Diagnostic Method of Acinetobacter baumannii and Acinetobacter Species Using Cell Wall Binding Domain of Bacteriophage Endolysin
한국미생물학회 학술대회논문집
2022 .10
-
Expression, purification, crystallization, and preliminary crystallographic analysis of the Fab fragment of golimumab, a therapeutic antibody against TNFα
Biodesign
2017 .06
-
Purification, crystallization and X-ray crystallographic analysis of methylmalonyl- CoA epimerase from Metallosphaera sedula
Biodesign
2021 .01
-
Overexpression, crystallization, and preliminary X-ray crystallographic analysis of a putative Diguanylate cyclase from Thermotoga maritima by the Tm0107 gene
Biodesign
2017 .06
-
Purification, crystallization and X-ray crystallographic analysis of mesodiaminopimelic acid decarboxylase from Corynebacterium glutamicum
Biodesign
2017 .06
-
Comparative Genomic Analysis of Emerging Extensively Drug-resistant Acinetobacter baumannii Strains in South Korea
한국미생물학회 학술대회논문집
2018 .04
-
Purification, crystallization, and X-ray crystallographic analysis of histidine triad nucleotide-binding protein from Candida albicans
Biodesign
2018 .01
-
Purification, crystallization, and X-ray crystallographic analysis of Nit2 from Kluyveromyces lactis
Biodesign
2020 .01
-
Deformation mechanisms of phases crystallizing from the melt during shearing
대한지질학회 학술대회
2022 .10
-
다제내성 Acinetobacter baumannii의 항생제 내성 유전자 분석
대한임상검사과학회지
2016 .01
-
Preliminary crystallographic analysis of the Fab fragment of camrelizumab, a therapeutic antibody against PD-1
Biodesign
2019 .01
-
Identification of Novel Antibiotic Resistance Genes in Environmental Bacteria
한국미생물학회 학술대회논문집
2021 .08
-
Biodegradation of Diesel Oil and n-Alkanes (C18, C20, and C22) by Novel Strain Acinetobacter sp. K-6 in Unsaturated Soil
한국미생물학회 학술대회논문집
2019 .04
이 논문의 저자 정보
최근 본 자료
댓글(0)
0