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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Background: Oxidative stress is a known to be associated with in the pathogenesis of many inflammatory diseases, including periodontitis. Ursolic acid is a pentacyclic triterpenoid with has antimicrobial, antioxidative, and anticancer properties. However, the role of ursolic acid in the regulating of osteogenesis remains undetermined. This study was aimed to elucidate the crucial osteogenic effects of ursolic acid and its ability to inhibit oxidative stress by targeting the immediate early response 3 (IER3)/nuclear factor erythroid 2-related factor 2 (Nrf2) pathway. Methods: Cell proliferation was determined using water-soluble tetrazolium salt assay, cell differentiation was evaluated by alkaline phosphatase (ALP) activity, and formation of calcium nodules was detected using alizarin red S stain. Generation of reactive oxygen species (ROS) was determined using by DCFH-DA fluorescence dye in hydrogen peroxide (H2O2)-treated MG-63 cells. Expression levels of IER3, Nrf2, and heme oxygenase-1 (HO-1) were analyzed using western blot analysis. Results: Our results showed that ursolic acid up-regulated the proliferation of osteoblasts without any cytotoxic effects, and promoted ALP activity and mineralization. H2O2-induced ROS generation was found to be significantly inhibited on treatment with ursolic acid. Furthermore, in H2O2-treated cells, the expression of the early response genes: IER3, Nrf2, and Nrf2-related phase II enzyme (HO-1) was enhanced in the presence of ursolic acid. Conclusion: The key findings of the present study elucidate the protective effects of ursolic acid against oxidative stress conditions in osteoblasts via the IER3/Nrf2 pathway. Thus, ursolic acid may be developed as a preventative and therapeutic agent for mineral homeostasis and inflammatory diseases caused due to oxidative injury.
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참고문헌 (28)
참고문헌 신청
Pizzino G / 2017 / Oxidative stress:harms and benefits for human health / Oxid Med Cell Longev :8416763
Cristina Espinosa-Diez / 2015 / Antioxidant responses and cellular adjustments to oxidative stress / Redox Biology 6:183~197
Manish Mittal / 2014 / Reactive Oxygen Species in Inflammation and Tissue Injury / Antioxidants & Redox Signaling 20(7):1126~1167
Okezie I. Aruoma / 1998 / Free radicals, oxidative stress, and antioxidants in human health and disease / Journal of the American Oil Chemists' Society 75(2):199~212
Stavros C. Manolagas / 2010 / From Estrogen-Centric to Aging and Oxidative Stress: A Revised Perspective of the Pathogenesis of Osteoporosis / Endocrine Reviews 31(3):266~300
Cristina Espinosa-Diez / 2015 / Antioxidant responses and cellular adjustments to oxidative stress / Redox Biology 6:183~197
Manish Mittal / 2014 / Reactive Oxygen Species in Inflammation and Tissue Injury / Antioxidants & Redox Signaling 20(7):1126~1167
Okezie I. Aruoma / 1998 / Free radicals, oxidative stress, and antioxidants in human health and disease / Journal of the American Oil Chemists' Society 75(2):199~212
Stavros C. Manolagas / 2010 / From Estrogen-Centric to Aging and Oxidative Stress: A Revised Perspective of the Pathogenesis of Osteoporosis / Endocrine Reviews 31(3):266~300
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