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논문 기본 정보

자료유형
학술저널
저자정보
Woo-Kon Lee (Gyeongsang National University School of Medicine) Seung-Chul Baik (Gyeongsang National University School of Medicine) Min-Kyung Shin (Gyeongsang National University School of Medicine) Myunghwan Jung (Gyeongsang National University School of Medicine) Jin-Sik Park (Gyeongsang National University School of Medicine) Jong-Hoon Ha (Gyeongsang National University School of Medicine) Dong-Hae Lee (Gyeongsang National University School of Medicine) Min-Jeong Kim (Gyeongsang National University School of Medicine) Jeong-ih Shin (Gyeongsang National University School of Medicine) Hyung-Lyun Kang (Gyeongsang National University School of Medicine)
저널정보
대한미생물학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY JOURNAL OF BACTERIOLOGY AND VIROLOGY Vol.49 No.4
발행연도
2019.12
수록면
191 - 202 (12page)

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In order to investigate the antioxidant effect of alkylhydroxide peroxidase (ahpC) of Helicobacter pylori (H. pylori) 26695, an ahpC-deficient mutant (H. pylori 26695 ahpC::cat) was generated. ahpC-deficient mutant was grown slowly at lower pressure of oxygen (5% oxygen) compared to the H. pylori 26695. Whole cell proteins isolated form H. pylori 26695 and H. pylori 26695 ahpC::cat were analyzed by MALDI-TOF and tandem-MS. The expression of 15 proteins, including Ppa, HypB, GrpE, Elp, RecA, GroES, Mda66, RibE, NapA, GlnA, BioB, TrxB, Tsf, FumC and Icd, was more than doubled in H. pylori 26695 ahpC::cat. Production of 10 proteins such as UreG, FabE, Adk, Pnp, OorC, AtpA, AtpD, Nqq3, Pfr, and TagD decreased below 50% in H. pylori 26695 ahpC::cat compared to the H. pylori 26695. In microarray analysis, 9 genes including sul1, amiE, frxA, fecA, hyuA, and katA increased in transcription level in H. pylori 26695 ahpC::cat compared to H. pylori 26695. A total of 24 genes, including flaB, protein kinase C inhibitor, cag16, pabC, and sabA, reduced in transcription. 27 genes, including HP0889, showed common expression changes in ahpC, katA, and sodB-deficient mutations. As a result of this study, there were not many genes whose expression was commonly changed by the deletion of each of the three major antioxidant enzymes of H. pylori. These results showed the functions and regulation of the three antioxidant enzymes were different in H. pylori.

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