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논문 기본 정보

자료유형
학술저널
저자정보
Sang Ah Yi (Sungkyunkwan University) Jieun Lee (Sungkyunkwan University) Sun Kyu Park (Green Cross WellBeing) Jeom Yong Kim (Green Cross WellBeing) Jong Woo Park (Sungkyunkwan University) Min Gyu Lee (Sungkyunkwan University) Ki Hong Nam (Sungkyunkwan University) Jee Hun Park (Sungkyunkwan University) Hwamok Oh (Sungkyunkwan University) Saetbyul Kim (Sungkyunkwan University) Jihoon Han (Sungkyunkwan University) Bo Kyung Kim (Sungkyunkwan University) Dong-Gyu Jo (Sungkyunkwan University) Jeung-Whan Han (Sungkyunkwan University)
저널정보
고려인삼학회 Journal of Ginseng Research Journal of Ginseng Research Vol.44 No.1
발행연도
2020.1
수록면
58 - 66 (9page)

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Background: The biological and pharmacological effects of BST204, a fermented ginseng extract, have been reported in various disease conditions. However, its molecular action in metabolic disease remains poorly understood. In this study, we identified the antiadipogenic activity of BST204 resulting from its inhibition of the S6 kinase 1 (S6K1) signaling pathway.
Methods: The inhibitory effects of BST204 on S6K1 signaling were investigated by immunoblot, nuclear fractionation, immunoprecipitation analyses. The antiadipogenic effect of BST204 was evaluated by measuring mRNA levels of adipogenic genes and by chromatin immunoprecipitation and quantitative real-time polymerase chain reaction analysis.
Results: Treatment with BST204 inhibited activation and nuclear translocation of S6K1, further decreasing the interaction between S6K1 and histone H2B in 10T1/2 mesenchymal stem cells. Subsequently, phosphorylation of H2B at serine 36 (H2BS36p) by S6K1 was reduced by BST204, inducing an increase in the mRNA expression of Wnt6, Wnt10a, and Wnt10b, which disturbed adipogenic differentiation and promoted myogenic and early osteogenic gene expression. Consistently, BST204 treatment during adipogenic commitment suppressed the expression of adipogenic marker genes and lipid drop formation.
Conclusion: Our results indicate that BST204 blocks adipogenesis of mesenchymal stem cells through the inhibition of S6K1-mediated histone phosphorylation. This study suggests the potential therapeutic strategy using BST204 to combat obesity and musculoskeletal diseases.

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ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References

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UCI(KEPA) : I410-ECN-0101-2020-524-000504657