지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 58 - 66 (9page)
이용수
초록· 키워드
오류제보하기
Background: The biological and pharmacological effects of BST204, a fermented ginseng extract, have been reported in various disease conditions. However, its molecular action in metabolic disease remains poorly understood. In this study, we identified the antiadipogenic activity of BST204 resulting from its inhibition of the S6 kinase 1 (S6K1) signaling pathway.
Methods: The inhibitory effects of BST204 on S6K1 signaling were investigated by immunoblot, nuclear fractionation, immunoprecipitation analyses. The antiadipogenic effect of BST204 was evaluated by measuring mRNA levels of adipogenic genes and by chromatin immunoprecipitation and quantitative real-time polymerase chain reaction analysis.
Results: Treatment with BST204 inhibited activation and nuclear translocation of S6K1, further decreasing the interaction between S6K1 and histone H2B in 10T1/2 mesenchymal stem cells. Subsequently, phosphorylation of H2B at serine 36 (H2BS36p) by S6K1 was reduced by BST204, inducing an increase in the mRNA expression of Wnt6, Wnt10a, and Wnt10b, which disturbed adipogenic differentiation and promoted myogenic and early osteogenic gene expression. Consistently, BST204 treatment during adipogenic commitment suppressed the expression of adipogenic marker genes and lipid drop formation.
Conclusion: Our results indicate that BST204 blocks adipogenesis of mesenchymal stem cells through the inhibition of S6K1-mediated histone phosphorylation. This study suggests the potential therapeutic strategy using BST204 to combat obesity and musculoskeletal diseases.
Methods: The inhibitory effects of BST204 on S6K1 signaling were investigated by immunoblot, nuclear fractionation, immunoprecipitation analyses. The antiadipogenic effect of BST204 was evaluated by measuring mRNA levels of adipogenic genes and by chromatin immunoprecipitation and quantitative real-time polymerase chain reaction analysis.
Results: Treatment with BST204 inhibited activation and nuclear translocation of S6K1, further decreasing the interaction between S6K1 and histone H2B in 10T1/2 mesenchymal stem cells. Subsequently, phosphorylation of H2B at serine 36 (H2BS36p) by S6K1 was reduced by BST204, inducing an increase in the mRNA expression of Wnt6, Wnt10a, and Wnt10b, which disturbed adipogenic differentiation and promoted myogenic and early osteogenic gene expression. Consistently, BST204 treatment during adipogenic commitment suppressed the expression of adipogenic marker genes and lipid drop formation.
Conclusion: Our results indicate that BST204 blocks adipogenesis of mesenchymal stem cells through the inhibition of S6K1-mediated histone phosphorylation. This study suggests the potential therapeutic strategy using BST204 to combat obesity and musculoskeletal diseases.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (51)
참고문헌 신청
Ma XM / 2009 / Molecular mechanisms of mTOR-mediated translational control / Nat Rev Mol Cell Biol 10:307~318
Ben-Sahra I / 2013 / Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1 / Science 339:1323~1328
Robitaille AM / 2013 / Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis / Science 339:1320~1323
Owen JL / 2012 / Insulin stimulation of SREBP-1c processing in transgenic rat hepatocytes requires p70 S6-kinase / Proc Natl Acad Sci USA 109:16184~16189
Um SH / 2004 / Absence of S6K1 protects against ageand diet-induced obesity while enhancing insulin sensitivity / Nature 431:200~205
Ben-Sahra I / 2013 / Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1 / Science 339:1323~1328
Robitaille AM / 2013 / Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis / Science 339:1320~1323
Owen JL / 2012 / Insulin stimulation of SREBP-1c processing in transgenic rat hepatocytes requires p70 S6-kinase / Proc Natl Acad Sci USA 109:16184~16189
Um SH / 2004 / Absence of S6K1 protects against ageand diet-induced obesity while enhancing insulin sensitivity / Nature 431:200~205
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
반하사심탕 혈맥약침이 흰쥐의 CINV(Chemotherapy-Induced Nausea and Vomiting)에 미치는 영향
대한한의학회지
2017 .03
-
Baumann Skin Type in the Korean Female Population
Annals of Dermatology
2017 .01
-
Decreased absorption of midazolam in the stomach due to low pH induced by coadministration of Banha-sasim-tang
Environmental Analysis Health and Toxicology
2016 .12
-
기침에 대한 반하사심탕의 효과 : 체계적인 문헌고찰과 메타분석
대한한방내과학회지
2021 .01
-
부자사심탕(附子瀉心湯)이 산화적 손상, 염증 및 골관절염 병태모델에 미치는 영향
한방재활의학과학회지
2015 .01
-
Efficiency of Computerized Insulin Infusion Glucose Control in Critically Ill Patients
Journal of Acute Care Surgery
2020 .01
-
Natural Products Targeting Wnt/β-catenin Signaling Pathway
Natural Product Sciences
2020 .06
-
Wnt signaling in liver regeneration, disease, and cancer
Clinical and Molecular Hepatology
2023 .01
-
상엽(桑葉) 추출물의 Adipogenesis 억제를 통한 항비만 활성 평가
대한본초학회지(본초분과학회지)
2016 .01
-
인삼 다당체의 항비만 활성 평가 및 기능성 소재 개발
대한본초학회지(본초분과학회지)
2016 .01
-
표준 치료를 받고 있는 제2형 당뇨병 환자에 대한 구미강활탕, 평위산, 반하사심탕 복용이 혈당에 미치는 영향 및 안전성 연구
대한한방내과학회지
2022 .06
-
Clinical Significance of Aberrant Wnt7a Promoter Methylation in Human Non-Small Cell Lung Cancer in Koreans
Journal of Korean Medical Science
2015 .01
-
Regulation of Osteoblast Metabolism by Wnt Signaling
Endocrinology and Metabolism
2018 .01
-
홍화 추출물이 생쥐 골수 유래 중간엽 줄기세포의 지방분화에 미치는 영향
대한한방내과학회지
2017 .01
-
Optimization of adipogenic differentiation conditions for canine adipose-derived stem cells
Journal of Veterinary Science
2021 .07
-
Wnt/β-catenin 신호 활성화를 통한 미세전류 자극의 지방생성 억제 효과
의공학회지
2020 .01
-
Decline in SESN2 Expression During Aging Shifts Mesenchymal Stem Cell Differentiation from Osteogenic to Adipogenic Lineage
International Journal of Oral Biology
2024 .09
-
Anti-adipogenic effect of Artemisia annua in diet-induced-obesity mice model
Journal of Veterinary Science
2015 .12
-
Anti-adipogenic Effect of Extract and Fractions of Cherry (Prunus serrulata var. spontanea) stem in 3T3-L1 Adipocytes
한국실험동물학회 학술발표대회 논문집
2015 .08
-
Wnt/β-Catenin Small-Molecule Inhibitor CWP232228 Preferentially Inhibits the Growth of Breast Cancer Stem-like Cells
한국실험동물학회 학술발표대회 논문집
2015 .08
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2020-524-000504657