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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제3호
- 발행연도
- 2020.1
- 수록면
- 459 - 468 (10page)
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초록· 키워드
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This study established a new polymerase spiral reaction (PSR) that combines with reverse transcription reactions for HCV detection targeting 5’UTR gene. To avoid cross-contamination of aerosols, an isothermal amplification tube (IAT), as a separate containment control, was used to judge the result. After optimizing the RT-PSR reaction system, its effectiveness and specificity were tested against 15 different virus strains which included 8 that were HCV positive and 7 as non-HCV controls. The results showed that the RT-PSR assay effectively detected all 8 HCV strains, and no false positives were found among the 7 non-HCV strains. The detection limit of our RT-PSR assay is comparable to the real-time RT-PCR, but is more sensitive than the RT-LAMP. The established RT-PSR assay was further evaluated for detection of HCV in clinical blood samples, and the resulting 80.25% detection rate demonstrated better or similar effectiveness compared to the RT-LAMP (79.63%) and real-time RT-PCR (80.25%). Overall, the results showed that the RT-PSR assay offers high specificity and sensitivity for HCV detection with great potential for screening HCV in clinical blood samples.
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참고문헌 (25)
참고문헌 신청
Brass V / 2006 / Molecular virology of hepatitis C virus(HCV) : 2006 update / Int. J. Med. Sci 3:29~34
Moradpour D / 2007 / Replication of hepatitis C virus / Nat. Rev. Microbiol 5:453~463
Messina JP / 2015 / Global distribution and prevalence of hepatitis C virus genotypes / Hepatology 61:77~87
Campo DS / 2016 / Accurate genetic detection of Hepatitis C Virus transmissions in outbreak settings / J. Infect. Dis 213:957~965
Chen Y / 2017 / Hepatitis C virus genotypes and subtypes circulating in Mainland China / Emerg. Microbes Infect 6(11):e95
Moradpour D / 2007 / Replication of hepatitis C virus / Nat. Rev. Microbiol 5:453~463
Messina JP / 2015 / Global distribution and prevalence of hepatitis C virus genotypes / Hepatology 61:77~87
Campo DS / 2016 / Accurate genetic detection of Hepatitis C Virus transmissions in outbreak settings / J. Infect. Dis 213:957~965
Chen Y / 2017 / Hepatitis C virus genotypes and subtypes circulating in Mainland China / Emerg. Microbes Infect 6(11):e95
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