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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 500 - 508 (9page)
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초록· 키워드
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Purpose: Lung adenocarcinoma (LA) is one of the major types of lung cancer. MicroRNAs (miRNAs) play an essential role in regulatingresponses of natural killer (NK) cells to cancer malignancy. However, the mechanism of miR-218-5p involved in the killingeffect of NK cells to LA cells remains poorly understood. Materials and Methods: The expression of miR-218-5p was examined by quantitative real-time polymerase chain reaction (qRTPCR). Serine hydroxymethyl transferase 1 (SHMT1) level was detected by qRT-PCR or western blots. Cytokines production ofinterferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) were detected by ELISA. The killing effect of NK cells to LA cells was investigatedusing lactate dehydrogenase cytotoxicity assay kit. The interaction of miR-218-5p and SHMT1 was probed by luciferaseactivity assay. Xenograft model was established to investigate the killing effect of NK cells in vivo. Results: miR-218-5p was enhanced and SHMT1 was inhibited in NK cells of LA patients, whereas stimulation of interleukin-2(IL-2) reversed their abundances. Addition of miR-218-5p reduced IL-2-induced cytokines expression and cytotoxicity in NK-92against LA cells. Moreover, SHMT1 was negatively regulated by miR-218-5p and attenuated miR-218-5p-mediated effect on cytotoxicity,IFN-γ and TNF-α secretion in IL-2-activated NK cells. In addition, miR-218-5p exhaustion inhibited tumor growth bypromoting killing effect of NK cells. Conclusion: miR-218-5p suppresses the killing effect of NK cells to LA cells by targeting SHMT1, providing a potential target forLA treatment by ameliorating NK cells function.
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참고문헌 (36)
참고문헌 신청
Global Burden of Disease Cancer Collaboration / 2017 / Global, regional, and national cancer incidence, mortality, years of life lost, years lived with disability, and disability-adjusted lifeyears for 32 cancer groups, 1990 to 2015 : a systematic analysis for the global burden of disease study / JAMA Oncol 3:524~548
Inamura K / 2018 / Clinicopathological characteristics and mutations driving development of early lung adenocarcinoma : tumor initiation and progression / Int J Mol Sci 19:1259
Donington JS / 2018 / Progress in the management of early-stage non-small cell lung cancer in 2017 / J Thorac Oncol 13:767~778
Shroff GS / 2018 / Targeted therapy and immunotherapy in the treatment of non-small cell lung cancer / Radiol Clin orth Am 56:485~495
Aktas¸ ON / 2018 / Role of natural killer cells in lung cancer / J Cancer Res Clin Oncol 144:997~1003
Inamura K / 2018 / Clinicopathological characteristics and mutations driving development of early lung adenocarcinoma : tumor initiation and progression / Int J Mol Sci 19:1259
Donington JS / 2018 / Progress in the management of early-stage non-small cell lung cancer in 2017 / J Thorac Oncol 13:767~778
Shroff GS / 2018 / Targeted therapy and immunotherapy in the treatment of non-small cell lung cancer / Radiol Clin orth Am 56:485~495
Aktas¸ ON / 2018 / Role of natural killer cells in lung cancer / J Cancer Res Clin Oncol 144:997~1003
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