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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Post-transcriptional RNA processing and translational regulations are important steps for gene expression. To analyze the 5'UTR of psbA that enhances translation of the sweet protein monellin in chloroplasts, we cloned the monellin gene, with and without the psbA 5'UTR, into the chloroplast expression vector for chloroplast transformation. Transgenic plants were identified as being transplastomic via PCR and Southern blot analyses. We also observed non-specific recombination during tobacco chloroplast transformation. Analyses of the transcription patterns showed that intercistronic cleavage of the psbA mRNA 5' untranslated (UTR) region was functional at the mature stage, with the monocistronic mRNA of monellin increasing while its dicistronic mRNA decreased. Moreover, monellin accumulation accounted for $2.3\%$ of the total soluble protein at the mature stage, but only $1.3\%$ at the young stage in transplastomic lines that contained the 5'UTR of psbA. These results suggest that activation of the endonucleolytic cleavage of the psbA 5'UTR element depends on chloroplast developmental conditions, and that it enhances the accumulation of sweet protein monellin in those chloroplasts.
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