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S-Adenosylmethionine decarboxylase (SAMDC) activity and mRNA amount increase in a light-dependent manner, and the accumulation of SAMDC transcripts might be partially regulated by a circadian clock in tritordeum and Pharbitis nil. How-ever, no research has been reported on the circadian regulation of SAMDC activity and corresponding gene expression. Here, we determined that SAMDC activity and its mRNA accumulation changed diurnally in carnation leaves. To identify the promoter region responsible for this rhythmicity, we performed northern blot analysis using a GUS cDNA probe in. transgenic tobacco plants. The GUS gene was under the control of serial deletions of the 5'-flanking region of a genomic clone corresponding to CSDC9 (gCSDC9). The region between -754 and -274, which negatively regulated the accumulation of GUS mRNA, had two HD-Zip-2 binding sequences (TAATAATTA) that were found by PLACE analysis of the promoter region. Another region at 273bp upstream from the transcription initiation site was sufficient for diurnal expression of GUS, and contained the putative sites responsible for diurnal expression, i.e., the GT-1 consensus sequence (GGTAAT) and a sequence necessary for this circadian expression (CAACTTCATC).
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Characterization of Promoter and 5'-UTR of S-adenosylmethionine decarboxylase Gene in its Expression.
한국식물학회 학술발표대회
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Studies on the regulation of Carnation S-adenosylmethionine decarboxylase during petal development.
한국동물학회 학술대회
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Characterization of 5´-Flanking Region of Carnation Genomic Clone Encoding S-adenosylmethionine Decarboxylase.
한국동물학회 학술대회
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Characterization and Light-Induced Expression of the S-Adenosyl- methionine Decarboxylase Gene from Ipomoea nil
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Cloning of S-adenosylmethionine Decarboxylase Genomic DNA from Carnation
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Cloning of the Arginine Decarboxylase Gene from Carnation Flower and its Expression
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Development of a Screening System for Plant Defense-Inducing Agent using Transgenic Tobacco Plant with PR-la Promoter and GUS Gene
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