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- 자료유형
- 학술저널
- 저자정보
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- 2017.1
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Objectives : The fruit of Prunus mume Siebold & Zucc. has been used as an alternative medicine and functional food in Korea and Japan for preventive and therapeutic purposes. However, its molecular actions and mechanism on anti-inflammatory activity have not been clearly investigated. The aim of this study was to clarify the anti-inflammatory activity of the ethanol extract of P. mume fruit (EEPM) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells, and sought to understand the associated molecular mechanisms. Methods : Cytotoxicity was assessed by an MTT assay. The amount of nitric oxide (NO) production was determined by nitrite assay. The mRNA expression of inducible nitric oxide synthase (iNOS) was analyzed by RT-PCR. In addition, expression levels of iNOS, nuclear factor-erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) protein were detected by Western blotting. Results : Our data indicated that EEPM inhibited NO production in LPS-stimulated RAW264.7 cells in a concentration-dependent manner. At the mRNA and protein levels, EEPM suppressed LPS-induced iNOS expression. On the other hand, EEPM markedly enhanced HO-1 expression, which was associated with an induction and nuclear translocation of Nrf2. Moreover, the inhibitory effect of EEPM against LPS？induced NO production was significantly enhanced by hemin, a HO-1 inducer; however, EEPM's effect on the production of NO was abolished by zinc protoporphyrin IX, a HO-1 inhibitor. Conclusion : The results suggest that EEPM can act as a suppressor agent on NO production through an activation of Nrf2/HO-1 signaling pathway, and may be a promising candidate for the treatment of inflammatory diseases.
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참고문헌 (32)
참고문헌 신청
Lawrence T / 2002 / Anti-inflammatory lipid mediators and insights into the resolution of inflammation / Nat Rev Immunol 2(10):787~795
Shabbir A / 2016 / Discovery of new benzothiazine derivative as modulator of pro-and anti-inflammatory cytokines in rheumatoid Arthritis / Inflammation 39(6):1918~1929
Tzeng YL / 2002 / Endotoxin of Neisseria meningitides composed only of intact lipid A : Inactivation of the meningococcal 3-deoxy-D-manno-octulosonic acid transferase / J Bacteriol 184(9):2379~2388
Hou YC / 1999 / Current trends in the development of nitric oxide donors / Curr Pharm Design 5(6):417~441
Nussler AK / 1993 / Inflammation, immunoregulation, and inducible nitric oxide synthase / J Leukoc Biol 54(2):171~178
Shabbir A / 2016 / Discovery of new benzothiazine derivative as modulator of pro-and anti-inflammatory cytokines in rheumatoid Arthritis / Inflammation 39(6):1918~1929
Tzeng YL / 2002 / Endotoxin of Neisseria meningitides composed only of intact lipid A : Inactivation of the meningococcal 3-deoxy-D-manno-octulosonic acid transferase / J Bacteriol 184(9):2379~2388
Hou YC / 1999 / Current trends in the development of nitric oxide donors / Curr Pharm Design 5(6):417~441
Nussler AK / 1993 / Inflammation, immunoregulation, and inducible nitric oxide synthase / J Leukoc Biol 54(2):171~178
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