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Simultaneous Analysis for Veterinary Drug Residues in Honey by HPLC/MS/MS
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HPLC-MS/MS를 이용한 벌꿀 중 동물용의약품 동시분석방법 연구

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Type
Academic journal
Author
Kim, Jong-Hwa (경기도보건환경연구원 첨가물분석팀) Moon, Sun-Ea (경기도보건환경연구원 첨가물분석팀) Kim, Ki-Yu (경기도보건환경연구원 첨가물분석팀) Jung, You-Jung (경기도보건환경연구원 첨가물분석팀) Lee, Chang-Hee (경기도보건환경연구원 첨가물분석팀) Ku, Eun-Jung (경기도보건환경연구원 첨가물분석팀) Yoon, Mi-Hye (경기도보건환경연구원 첨가물분석팀) Lee, Jong-Bok (경기도보건환경연구원 첨가물분석팀)
Journal
한국식품위생안전성학회 한국식품위생안전성학회지 한국식품위생안전성학회지 제31권 제2호 KCI Accredited Journals
Published
2016.1
Pages
94 - 98 (5page)

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Simultaneous Analysis for Veterinary Drug Residues in Honey by HPLC/MS/MS
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This study was conducted to establish the simultaneous analysis method for veterinary drug residues in honey by high performance liquid chromatography tandem mass spectrometry (HPLC/MS/MS). The eleven targeting veterinary drugs with honey test method in Korean Food Standards Codex were divided into Group 1 (streptomycine dihydrostreptomycine, neomycine) and Group 2 (oxytetracycline, enrofloxacin, ciprofloxacin, cymiazole, chloramphenicol, amitraz, coumaphos, fluvalinate) to be analyzed simultaneously. From the results, the retention time (RT) of the targeting drugs was within 15 min, the range of detection limits was 0.0056 to $0.0643{\mu}g/g$ and the range of quantification limits was 0.0169 to $0.1948{\mu}g/g$. The coefficients of determination ($R^2$) for Group 1 ($0.05{\sim}1.0{\mu}g/mL$) and Group 2 ($0.01{\sim}1.0{\mu}g/mL$) were 0.9917~0.9987 and 0.9923~1.000 respectively, and showed the good linearity. The recovery rates for Group 1 (final conc. $0.25{\mu}g/g$) and Group 2 (final conc. $1.0{\mu}g/g$) were 65.1~80.6% and 64.2~90.3% respectively. Also, the analysis results of inter day (n = 3) and intra day (n = 6) RSD (%) for area and retention time showed that the RSD (%) for area and retention time was below 10.92% and 1.57%. Therefore, the simultaneous analysis method of this study is evaluated to be a good test method for veterinary drug residues in honey.

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