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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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We developed and subsequently characterized mouse monoclonal antibodies (MAbs) against recombinant VP28 structural protein (rVP28) of white spot syndrome virus (WSSV). We established six hybridoma clones secreting MAbs against rVP28: 15A11, 20G6, 31H2, 34H6, 38D1 and 43A1. All six MAbs recognized the 25 kDa of protein in gill homogenates of WSSV-infected shrimp by western blot analysis, while no reactivity was observed in gill homogenates of normal shrimp. Moreover, high enzyme-linked immunosorbent assay (ELISA) optical density (OD) values (0.8-2.68) were observed in the hemolymphs from WSSV-infected shrimp, while low OD values (less than 0.24) were recorded in the hemolymphs from normal shrimp, by using these six MAbs produced in this study. These results suggest that these six MAbs are useful for the detection of WSSV.
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참고문헌 (11)
참고문헌 신청
Egusa, S. / Infectious and parasitic diseases of fish and shellfish / Life Science Publishing Co :54~58
Gregorio, N.E. / 2019 / A user’s guide to cell-free protein synthesis / Methods Protoc 24:1~34
Jeong, H. N. / 2017 / Production of monoclonal antibodies against viral hemorrhagic septicemia virus (VHSV, genotype IVa)from olive flounder / J. Fish Pathol 30:149~154
김위식 / 2018 / 신경괴사증바이러스(nervous necrosis virus, RGNNV genotype)에 대한단클론 항체 생산 / 한국수산과학회지 51(3):328~331
Laemmli, U. K / 1970 / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 / Nature 227:680~685
Gregorio, N.E. / 2019 / A user’s guide to cell-free protein synthesis / Methods Protoc 24:1~34
Jeong, H. N. / 2017 / Production of monoclonal antibodies against viral hemorrhagic septicemia virus (VHSV, genotype IVa)from olive flounder / J. Fish Pathol 30:149~154
김위식 / 2018 / 신경괴사증바이러스(nervous necrosis virus, RGNNV genotype)에 대한단클론 항체 생산 / 한국수산과학회지 51(3):328~331
Laemmli, U. K / 1970 / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 / Nature 227:680~685
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