지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
The aim of this study was to evaluate effect of ${\alpha}$-linolenic acid (ALA) on viability, acrosome reaction and mitochondrial intact in frozen-thawed boar sperm. The boar semen was collected by gloved-hand method and cryopreserved in 20% egg yolk freezing extender containing ALA (0, 3, 5, and 10 ng/mL) with 0.05% ethanol. The frozen-boar spermatozoa were thawed at $37.5^{\circ}C$ for 45 sec in water-bath. The spermatozoa samples were evaluated the plasma membrane integrity, acrosome reaction, and mitochondrial integrity using flow cytometry. In results, population of live sperm with intact plasma membrane was significantly higher in control and 3 ng/mL ALA treatment group than ethanol group (p<0.05). In contract, dying sperms were higher in ethanol group than 3 ng/mL ALA treatment (p<0.05). Acrosomal membrane damage in all sperm population was reduced in 3 ng/mL ALA groups compared with ethanol treatment (p<0.05). However, acrosome damage in live sperm population was no significant difference among the all treatment groups. Mitochondrial integrity was not influenced by ALA treatments in both of live and all sperm population. In conclusion, this results show that supplement of ALA during the cryopreservation process could reduce the membrane damages including plasma and acrosomal membrane, whereas ALA did not influence to mitochondria in boar spermatozoa. Therefore, these results suggest that ALA can protect against the membrane damage derived cryo-stress, and cryopreservation efficiency of boar semen would be improved by use of ALA.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Effects of α-Linolenic Acid and Bovine Serum Albumin on Frozen-thawed Boar Sperm Quality during Cryopreservation
한국동물번식학회지
2016 .01
-
Low and High Levels of Exogenous 5-Aminolevulinic Acid Differentially Regulate Porphyrin Biosynthesis in Rice
Weed&Turfgrass Science
2020 .12
-
CRYOPRESERVATION OF STURGEON SPERM : EFFECTS OF EXTENDER SUPPLEMENTED WITH DIFFERENT ANTIOXIDANTS ON SPERM MOTILITY, MEMBRANE AND ACROSOME INTEGRITY
한국수산과학회 양식분과 학술대회
2015 .05
-
Antioxidant Effect of Alpha-Linolenic Acid during In Vitro Maturation in Porcine Oocytes
한국동물번식학회지
2017 .01
-
Attenuation of lipopolysaccharide-induced inflammatory and oxidative reaction by 5-aminolevulinic acid in RAW 264.7 macrophages and zebrafish larvae
한국수산과학회 양식분과 학술대회
2021 .04
-
Evaluation of Antifreeze Proteins on Miniature Pig Sperm Viability, DNA Damage, and Acrosome Status during Cryopreservation
한국수정란이식학회지
2016 .01
-
Alpha-linolenic acid regulates amyloid precursor protein processing by mitogen-activated protein kinase pathway and neuronal apoptosis in amyloid beta-induced SH-SY5Y neuronal cells
Applied Biological Chemistry
2018 .01
-
항산화 효소가 첨가된 Percoll에 의해 분리한 돼지 정액의 동결-융해 능력
한국수정란이식학회지
2015 .01
-
Effects of ice-binding protein from Leucosporidium on the cryopreservation of boar sperm
한국수정란이식학회지
2018 .01
-
Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using Protein- Chip CM10
한국축산학회지
2023 .03
-
Effects of L-Carnitine and Nicotinic Acid on Sperm Characteristics in Miniature Pigs
한국동물번식학회지
2016 .01
-
Comparison of Spermatozoa Recovery Methods on Cauda Epididymal Sperm of Hanwoo Bulls
한국수정란이식학회지
2018 .01
-
Motility, Fertilizability and Subsequent Embryonic Development of Frozen-thawed Spermatozoa derived from Epididymis in Hanwoo
한국수정란이식학회지
2015 .01
-
Effects of Turine and Vitamin E on Sperm Viability, Membrane Integrity and Mitochondrial Activity damaged by Bromopropane in Fresh Boar Semen
한국수정란이식학회지
2016 .01
-
Pentoxifylline treatment of frozen pig sperm affects sperm motility and fetal numbers
Korean Journal of Agricultural Science
2020 .09
-
Ameliorative Effect of Chitosan Complex on Miniature Pig Sperm Cryopreservation
한국수정란이식학회지
2018 .01
-
Effect of Optixcell and Triladyl extenders on frozen-thawed sperm motilities and calving rates following artificial insemination in Hanwoo
Korean Journal of Agricultural Science
2019 .03
-
Antioxidant Supplementation Enhances the Porcine Semen Preservation Capacity
한국동물번식학회지
2015 .01
-
Analysis of epididymal sperm from Korean native bull (Hanwoo) aged at 8 and 15 months before freezing and after thawing
한국수정란이식학회지
2016 .01
-
In vitro fertilization using sex-sorted boar sperm mediated by magnetic nanoparticles
Korean Journal of Agricultural Science
2020 .12
이 논문의 저자 정보
최근 본 자료
댓글(0)
0