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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 193 - 200 (8page)
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Objectives: This study was performed to evaluate the neurototoxicity of the environmental pollutant lead acetate(LA) and the protective effect of the D-2-amino-5-phosphonovaleric acid(APV), N-methyl-D-aspartate(NMDA) receptor antagonist on LA-induced cytotoxicity in cultured C6 glioma cells. Materials and Methods: For this study, cell viability in cultured C6 glioma cells was assessed by XTT assay and antioxidative effect, such as lactate dehydrogenase(LDH) activity, by LDH detection kit. Results: LA significantly decreased cell viability in a dose-dependent manner, and the XTT50 value was determined to be 33.3 uM of LA. The cytotoxicity of LA was deemed highly toxic according to Borenfreund and Puerner's toxic criteria. The vitamin E antioxidant significantly increased cell viability damaged by LA-induced cytotoxicity in these cultures. For the protective effect of APV on LA-induced cytotoxicity, APV significantly increased not only cell viability, but also inhibition of LDH activity. From these results, it is suggested that oxidative stress is involved in the neurotoxicity of LA, and APV effectively protected against LA-induced cytotoxicity via an antioxidative effect as an inhibotory activity of LDH. Conclusions: Natural resources like APV may be putative therapeutic agents for the toxic diminution of environmental pollutants such as LA correlated with oxidative stress.
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참고문헌 (26)
참고문헌 신청
Bakker MH / 1991 / [3H]MK-801 binding to N-methyl-D-aspartate receptors sloubilized from rat brain: Effects of glycine site ligands, polyamines, ifenprodil, and desipramine / J Neurochem 57:39~45
Borenfreund E / 1984 / A simple quantitative procedure using monolayer culture for cytotoxicity assay(HTD/NR-90) / J Tiss Cult Meth 9(1):7~9
Bressler J / 1994 / Phospholipid metabolism in neural microvascular endothelial cells after exposure to lead in vitro / Toxicol Appl Pharmacol 126:352~360
Buchan A / 1994 / A selective N-type Ca2+ channel blocker prevent CA1 injury 24 hr following severe forebrain ischemia and reduces in fraction following focal ischemia / J Cereb Blood Flow Metab 14:903~910
Busselberg D / 1991 / Lead and zinc block a voltage-activated calcium channel of aplysia neurons / J Neurophysiol 65:786~795
Borenfreund E / 1984 / A simple quantitative procedure using monolayer culture for cytotoxicity assay(HTD/NR-90) / J Tiss Cult Meth 9(1):7~9
Bressler J / 1994 / Phospholipid metabolism in neural microvascular endothelial cells after exposure to lead in vitro / Toxicol Appl Pharmacol 126:352~360
Buchan A / 1994 / A selective N-type Ca2+ channel blocker prevent CA1 injury 24 hr following severe forebrain ischemia and reduces in fraction following focal ischemia / J Cereb Blood Flow Metab 14:903~910
Busselberg D / 1991 / Lead and zinc block a voltage-activated calcium channel of aplysia neurons / J Neurophysiol 65:786~795
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