YJI-7 Suppresses ROS Production and Expression of Inflammatory Mediators via Modulation of p38MAPK and JNK Signaling in RAW 264.7 Macrophages

Oh, Hye Jin; Magar, Til Bahadur Thapa; Pun, Nirmala Tilija; Lee, Yunji; Kim, Eun Hye; Lee, Eung-Seok; Park, Pil-Hoon

추천

검색

자료유형: 학술저널

저자정보: Oh, Hye Jin (College of Pharmacy, Yeungnam University) Magar, Til Bahadur Thapa (College of Pharmacy, Yeungnam University) Pun, Nirmala Tilija (College of Pharmacy, Yeungnam University) Lee, Yunji (College of Pharmacy, Yeungnam University) Kim, Eun Hye (College of Pharmacy, Yeungnam University) Lee, Eung-Seok (College of Pharmacy, Yeungnam University) Park, Pil-Hoon (College of Pharmacy, Yeungnam University)

저널정보: 한국응용약물학회 Biomolecules & Therapeutics(구 응용약물학회지) Biomolecules & therapeutics 제26권 제2호

발행연도: 2018.1

수록면: 191 - 200 (10page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Chalcone, (2E)-1,3-Diphenylprop-2-en-1-one, and its synthetic derivatives are known to possess anti-oxidative and anti-inflammatory properties. In the present study, we prepared a novel synthetic chalcone compound, (E)-1-(4-hydroxyphenyl)-3-(2-(trifluoromethoxy)phenyl)prop-2-en-1-one name (YJI-7), and investigated its inhibitory effects on endotoxin-stimulated production of reactive oxygen species (ROS) and expression of inflammatory mediators in macrophages. We demonstrated that treatment of RAW 264.7 macrophages with YJI-7 significantly suppressed lipopolysaccharide (LPS)-stimulated ROS production. We also found that YJI-7 substantially decreased NADPH oxidase activity stimulated by LPS, indicating that YJI-7 regulates ROS production via modulation of NADPH oxidase in macrophages. Furthermore, YJI-7 strongly inhibited the expression of a number of inflammatory mediators in a gene-selective manner, suggesting that YJI-7 possesses potent anti-inflammatory properties, as well as anti-oxidative activity. In continuing experiments to investigate the mechanisms that could underlie such biological effects, we revealed that YJI-7 suppressed phosphorylation of p38MAPK and JNK stimulated by LPS, whereas no significant effect on ERK was observed. Furthermore, LPS-stimulated production of ROS, activation of NADPH oxidase and expression of inflammatory mediators were markedly suppressed by treatment with selective inhibitor of p38MAPK (SB203580) and JNK (SP600125). Taken together, these results demonstrated that YJI-7, a novel synthetic chalcone derivative, suppressed LPS-stimulated ROS production via modulation of NADPH oxidase and diminished expression of inflammatory mediators, at least in part, via down-regulation of p38MAPK and JNK signaling in macrophages.

#Chalcone #NADPH oxidase #Reactive oxygen species #p38MAPK #JNK

참고문헌 (33)

참고문헌 신청

Ben-Baruch, A. / 1995 / Signals and receptors involved in recruitment of inflammatory cells / J. Biol. Chem. 270:11703~11706

Coussens, L. M. / 2002 / Inflammation and cancer / Nature 420:860~867

Eikelenboom, P. / 1994 / Inflammatory mechanisms in Alzheimer's disease / Trends Pharmacol. Sci. 15:447~450

Fischer, R. / 2015 / Interrelation of oxidative stress and inflammation in neurodegenerative disease: role of TNF / Oxid. Med. Cell. Longev. 2015:610813

Forejtnikova, H. / 2005 / Chemoprotective and toxic potentials of synthetic and natural chalcones and dihydrochalcones in vitro / Toxicology 208:81~93

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!