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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 441 - 449 (9page)
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초록· 키워드
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Objective : Notch receptors are heterodimeric transmembrane proteins that regulate cell fate, such as differentiation, proliferation, and apoptosis. Dysregulated Notch pathway signaling has been observed in glioblastomas, as well as in other human malignancies. Nerve growth factor (NGF) is essential for cell growth and differentiation in the nervous system. Recent reports suggest that NGF stimulates glioblastoma proliferation. However, the relationship between NGF and Notch1 in glioblastomas remains unknown. Therefore, we investigated expression of Notch1 in a glioblastoma cell line (U87-MG), and examined the relationship between NGF and Notch1 signaling. Methods : We evaluated expression of Notch1 in human glioblastomas and normal brain tissues by immunohistochemical staining. The effect of NGF on glioblastoma cell line (U87-MG) was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. To evaluate the relationship between NGF and Notch1 signaling, Notch1 and Hes1 expression were evaluated by reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. To confirm the effects of NGF on Notch1 signaling, Notch1 and Hes1 small interfering RNAs (siRNAs) were used. Results : In immunohistochemistry, Notch1 expression was higher in glioblastoma than in normal brain tissue. MTT assay showed that NGF stimulates U87-MG cells in a dose-dependent manner. RT-PCR and Western blot analysis demonstrated that Notch1 and Hes1 expression were increased by NGF in a dose-dependent manner. After transfection with Notch1 and Hes1 siRNAs, there was no significant difference between controls and 100 nM $NGF-{\beta}$, which means that U87-MG cell proliferation was suppressed by Notch1 and Hes1 siRNAs. Conclusion : These results indicate that NGF stimulates glioblastoma cell proliferation via Notch1 signaling through Hes 1.
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등록된 정보가 없습니다.
참고문헌 (40)
참고문헌 신청
Artavanis-Tsakonas S / 1999 / Notch signaling: cell fate control and signal integration in development / Science 284:770~776
Aster JC / 2001 / Notch signaling in leukemia / Curr Opin Hematol 8:237~244
Bigas A / 1998 / Notch1 and Notch2 inhibit myeloid differentiation in response to different cytokines / Mol Cell Biol 18:2324~2333
Bolos V / 2007 / Notch signaling in development and cancer / Endocr Rev 28:339~363
Brown MC / 2008 / Regulatory effect of nerve growth factor in alpha9beta1 integrin-dependent progression of glioblastoma / Neuro Oncol 10:968~980
Aster JC / 2001 / Notch signaling in leukemia / Curr Opin Hematol 8:237~244
Bigas A / 1998 / Notch1 and Notch2 inhibit myeloid differentiation in response to different cytokines / Mol Cell Biol 18:2324~2333
Bolos V / 2007 / Notch signaling in development and cancer / Endocr Rev 28:339~363
Brown MC / 2008 / Regulatory effect of nerve growth factor in alpha9beta1 integrin-dependent progression of glioblastoma / Neuro Oncol 10:968~980
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