지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제17권 제3호
- 발행연도
- 2016.1
- 수록면
- 1,499 - 1,506 (8page)
이용수
초록· 키워드
오류제보하기
Background: Crocus sativus and its major constituent crocin are well established to have anti-cancer properties in breast cancer cells (MCF-7). However the role of C. sativus extract (CSE) and crocin on caspase signaling mediated MCF-7 cell death at molecular level is remains unclear. In this study, we tried to unravel role of CSE and crocin on caspase mediated MCF-7 cells death and their in vivo preclinical toxicity profiling and immune stimulatory effect. Materials and Methods: CSE extract was fractionated by HPLC and crocin was isolated and characterized by NMR, IR, and MS. MCF-7 cells were treated with both CSE and crocin and expression of Bcl-2 and Bax was assessed after 24 and 36 hours. Furthermore, caspase 3, caspase 8 and caspase 9 expression was determined by Western blotting after 24 hours of treatment. DNA fragmentation analysis was performed for genotoxicity of CSE and crocin in MCF-7 cells. The in vivo toxicity profile of CSE (300 mg/kg of b.wt) was investigated in normal Swiss albino mice. In addition, peritoneal macrophages were collected from crocin (1, 1.5 and 2 mg/kg body weight) treated mice and analyzed for ex vivo yeast phagocytosis. Results: Immunoblot analysis revealed that there was time dependent decline in anti-apoptotic Bcl-2 with simultaneous upregulation of Bax in CSE and crocin treated MCF-7 cells. Further CSE and crocin treatment downregulated caspase 8 and 9 and cleaved the caspase 3 after 24 hours. Both CSE and crocin elicited considerable DNA damage in MCF-7 cells at each concentration tested. In vivo toxicity profile by histological studies revealed no observable histopathologic differences in the liver, kidney, spleen, lungs and heart in CSE treated and untreated groups. Crocin treatment elicited significant dose and time dependent ex vivo yeast phagocytosis by peritoneal macrophages. Conclusions: Our study delineated involvement of pro-apoptotic and caspase mediated MCF-7 cell death by CSE and crocin at the molecular level accompanied with extensive DNA damage. Further we found that normal swiss albino mice can tolerate the maximum dose of CSE. Crocin enhanced ex vivo macrophage yeast phagocytic ability.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Preventive effects of crocin on neuronal damages induced by D-galactose through AGEs and oxidative stress in human neuroblastoma cells (SH-SY5Y)
Journal of Pharmacopuncture
2018 .01
-
The Prophylactic and Therapeutic Effects of Saffron Extract and Crocin on Ethanol Withdrawal Syndrome in Mice
Journal of Pharmacopuncture
2018 .01
-
Crocin Improves Oxidative Stress by Potentiating Intrinsic Anti-Oxidant Defense Systems in Pancreatic Cells During Uncontrolled Hyperglycemia
Journal of Pharmacopuncture
2019 .01
-
Apoptotic Effects of 6-Gingerol in Human Breast Cancer Cells
International Journal of Oral Biology
2015 .01
-
Cytotoxic Effect of Triglycerides via Apoptotic Caspase Pathway in Immune and Non-immune Cell Lines
대한의생명과학회지
2019 .03
-
담배연기에 의한 선천성 면역반응 자극 및 천식의 발생과 악화
Allergy Asthma & Respiratory Diseases
2022 .07
-
Evaluation Expression of the Caspase-3 and Caspase-9 Apoptotic Genes in Schizophrenia Patients
Clinical Psychopharmacology and Neuroscience
2023 .02
-
Comparative Evaluation of Hepatoprotective Activities of Geniposide, Crocins and Crocetin by CCl<sub>4</sub>-Induced liver Injury in Mice
Biomolecules & Therapeutics(구 응용약물학회지)
2016 .01
-
Isocryptotanshinone Induced Apoptosis and Activated MAPK Signaling in Human Breast Cancer MCF-7 Cells
Journal of Breast Cancer
2015 .01
-
The Phosphodiesterase 4 Inhibitor Roflumilast Protects against Cigarette Smoke Extract-Induced Mitophagy-Dependent Cell Death in Epithelial Cells
Tuberculosis and Respiratory Diseases
2018 .01
-
3T3L-1 지방전구세포와 고지방식이로 유도된 비만 마우스 모델에서 모과 추출물의 항비만 효과와 억제 기전
대한한의학방제학회지
2017 .01
-
Momordica cochinchinensis Aril Extract Induced Apoptosis in Human MCF-7 Breast Cancer Cells
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Comparative Evaluation of Silibinin Effects on Cell Cycling and Apoptosis in Human Breast Cancer MCF-7 and T47D Cell Lines
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Effects of Triterpenoids from Luvunga scandens on Cytotoxic, Cell Cycle Arrest and Gene Expressions in MCF-7 Cells
Natural Product Sciences
2016 .12
-
Phytochemicals from Goniothalamus griffithii Induce Human Cancer Cell Apoptosis
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
참옻 추출물의 MCF-7 인체 유방암 세포에서 증식 억제효과
대한한의학방제학회지
2016 .01
-
한국어판 피임 자기효능감(K-CSE) 도구의 신뢰도와 타당도: 여대생을 중심으로
Women's Health Nursing
2016 .01
-
15d-PGJ2 Induces Apoptosis of MCF-7 and MDA-MB-231 Cells via Increased Intracellular Calcium and Activation of Caspases, Independent of ERα and ERβ
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Comparison of in Vitro Cytotoxicity and Apoptogenic Activity of Magnesium Chloride and Cisplatin as Conventional Chemotherapeutic Agents in the MCF-7 Cell Line
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
An updated review on therapeutic effects of nanoparticle-based formulations of saffron components (safranal, crocin, and crocetin)
Journal of Pharmaceutical Investigation
2020 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0