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논문 기본 정보

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학술저널
저자정보
Yoon, Ji-Young (Department of Dental Anesthesia and Pain Medicine, School of Dentistry, Pusan National University, Dental Research Institute) Baek, Chul-Woo (Department of Dental Anesthesia and Pain Medicine, School of Dentistry, Pusan National University, Dental Research Institute) Kim, Eun-Jung (Department of Dental Anesthesia and Pain Medicine, School of Dentistry, Pusan National University, Dental Research Institute) Park, Bong-Soo (Department of Oral Anatomy, School of Dentistry, Pusan National University) Yu, Su-Bin (Department of Oral Anatomy, School of Dentistry, Pusan National University) Yoon, Ji-Uk (Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University) Kim, Eok-Nyun (Department of Dental Anesthesia and Pain Medicine, School of Dentistry, Pusan National University, Dental Research Institute)
저널정보
대한치과마취과학회 Journal of dental anesthesia and pain medicine Journal of dental anesthesia and pain medicine 제17권 제1호
발행연도
2017.1
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37 - 46 (10page)

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Background: In oxidative stress, reactive oxygen species (ROS) production contributes to cellular dysfunction and initiates the apoptotic cascade. Autophagy is considered the mechanism that decreases ROS concentration and oxidative damage. Propofol shows antioxidant properties, but the mechanisms underlying the effect of propofol preconditioning (PPC) on oxidative injury remain unclear. Therefore, we investigated whether PPC protects against cell damage from hydrogen peroxide ($H_2O_2$)-induced oxidative stress and influences cellular autophagy. Method: COS-7 cells were randomly divided into the following groups: control, cells were incubated in normoxia (5% $CO_2$, 21% $O_2$, and 74% $N_2$) for 24 h without propofol; $H_2O_2$, cells were exposed to $H_2O_2$ ($400{\mu}M$) for 2 h; $PPC+H_2O_2$, cells pretreated with propofol were exposed to $H_2O_2$; and 3-methyladenine $(3-MA)+PPC+H_2O_2$, cells pretreated with 3-MA (1 mM) for 1 h and propofol were exposed to $H_2O_2$. Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide thiazolyl blue (MTT) reduction. Apoptosis was determined using Hoechst 33342 staining and fluorescence microscopy. The relationship between PPC and autophagy was detected using western blot analysis. Results: Cell viability decreased more significantly in the $H_2O_2$ group than in the control group, but it was improved by PPC ($100{\mu}M$). Pretreatment with propofol effectively decreased $H_2O_2$-induced COS-7 cell apoptosis. However, pretreatment with 3-MA inhibited the protective effect of propofol during apoptosis. Western blot analysis showed that the level of autophagy-related proteins was higher in the $PPC+H_2O_2$ group than that in the $H_2O_2$ group. Conclusion: PPC has a protective effect on $H_2O_2$-induced COS-7 cell apoptosis, which is mediated by autophagy activation.
#Autophagy #COS-7 Cells #Oxidative Stress #Propofol

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참고문헌 (37)

참고문헌 신청
Finkel T / 2000 / Oxidants, oxidative stress and the biology of ageing / Nature 408:239~247 google schola Geiszt M / 2000 / Identification of renox, an NAD(P)H oxidase in kidney / Proc Natl Acad Sci U S A 97:8010~8014 google schola Boveris A / 1973 / The mitochondrial generation of hydrogen peroxide. general properties and effect of hyperbaric oxygen / Biochem J 134:707~716 google schola Chien CT / 2001 / De novo demonstration and co-localization of freeradical production and apoptosis formation in rat kidney subjected to ischemia/reperfusion / J Am Soc Nephrol 12:973~982 google schola Filomeni G / 2015 / Oxidative stress and autophagy : The clash between damage and metabolic needs / Cell Death Differ 22:377~388 google schola

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