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자료유형
학술저널
저자정보
Oh, Sangnam (BK21 Plus Graduate Program, Department of Animal Science and Institute of Agricultural Science & Technology, Chonbuk National University) Park, Mi Ri (BK21 Plus Graduate Program, Department of Animal Science and Institute of Agricultural Science & Technology, Chonbuk National University) Son, Seok Jun (BK21 Plus Graduate Program, Department of Animal Science and Institute of Agricultural Science & Technology, Chonbuk National University) Kim, Younghoon (BK21 Plus Graduate Program, Department of Animal Science and Institute of Agricultural Science & Technology, Chonbuk National University)
저널정보
한국축산식품학회 한국축산식품학회지 한국축산식품학회지 제35권 제4호
발행연도
2015.1
수록면
459 - 465 (7page)

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Bovine milk provides essential nutrients, including immunologically important molecules, as the primary source of nutrition to newborns. Recent studies showed that RNAs from bovine milk contain immune-related microRNAs (miRNA) that regulate various immune systems. To evaluate the biological and immunological activity of miRNAs from milk products, isolation methods need to be established. Six methods for extracting total RNAs from bovine colostrums were adopted to evaluate the isolating efficiency and expression of miRNAs. Total RNA from milk was presented in formulation of small RNAs, rather than ribosomal RNAs. Column-combined phenol isolating methods showed high recovery of total RNAs, especially the commercial columns for biofluid samples, which demonstrated outstanding efficiency for recovering miRNAs. We also evaluated the quantity of five immune-related miRNAs (miR-93, miR-106a, miR-155, miR-181a, miR-451) in milk processed by temperature treatments including low temperature for long time (LTLT, 63℃ for 30 min)-, high temperature for short time (HTST, 75℃ for 15 s)-, and ultra heat treatment (UHT, 120-130℃ for 0.5-4 s). All targeted miRNAs had significantly reduced levels in processed milks compared to colostrum and raw mature milk. Interestingly, the amount of immune-related miRNAs from HTST milk was more resistant than those of LTLT and UHT milks. Our present study examined defined methods of RNA isolation and quantification of immune-specific miRNAs from small volumes of milk for use in further analysis.

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