Optimization of Post-Activation Systems to Improve the Embryonic Development in Porcine Parthenogenesis and Somatic Cell Nuclear Transfer

Roy, Pantu Kumar; Kim, Ghangyong; Fang, Xun; Hassan, Bahia MS; Soysa, Mahanama De; Shin, Sang Tae; Cho, Jong Ki

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자료유형: 학술저널

저자정보: Roy, Pantu Kumar (College of Veterinary Medicine, Chungnam National University) Kim, Ghangyong (College of Veterinary Medicine, Chungnam National University) Fang, Xun (College of Veterinary Medicine, Chungnam National University) Hassan, Bahia MS (College of Veterinary Medicine, Chungnam National University) Soysa, Mahanama De (College of Veterinary Medicine, Chungnam National University) Shin, Sang Tae (College of Veterinary Medicine, Chungnam National University) Cho, Jong Ki (College of Veterinary Medicine, Chungnam National University)

저널정보: 한국동물번식학회 한국수정란이식학회지 한국수정란이식학회지 제32권 제3호

발행연도: 2017.1

수록면: 95 - 104 (10page)

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This study was conducted to establish the optimal chemical post-activation conditions in porcine embryonic development after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT) using 4 different chemical compositions (cytochalasin B (CB), cyclohexamide (CHX), demecolcine (DC), 6-dimethylaminopurine (DMAP). Porcine embryos were produced by PA and SCNT and then, cultured for post-activation with CB ($7.5{\mu}g/mL$), CB ($7.5{\mu}g/mL$) + CHX ($10{\mu}g/mL$), CB ($7.5{\mu}g/mL$) +DC ($0.4{\mu}g/mL$), and CB ($7.5{\mu}g/mL$) + DMAP (2 mM). In PA embryonic development, cleavage rates have been significantly higher in CB group (94.7%) and CB+DMAP group (94.1%) than that of CB+CHX and CB+DC group (88.1 and 84.3%, respectively). There have been no significant differences in blastocyst formation rates among the four groups. In cell number of blastocyst was shown in CB group (42.3%) significantly higher than CB+CHX and CB+DC group (40.6 and 40.6%, respectively). In SCNT embryonic development, CB+DMAP group (89.7%) significant differences were found on embryo cleavage rates when compared with other three groups. Blastocyst formation rates in CB+DMAP group (26.9%) were significantly higher when compared with CB, CB+CHX, and CB+DC groups (25.5, 20.2, and 22.1%, respectively). In blastocyst cell number, CB+DMAP group (41.4%) was found higher significant difference compared with other three groups. Additionally, we have investigated survivin expression in early development stages of porcine SCNT embryos for more confirmation. Our results establish that CB group and CB+DMAP group for 4 h during post-activation improves pre-implantation improvement of PA and SCNT embryos.

#Embryo #Cloning #Parthenogenesis #Pig #Post-activation

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