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논문 기본 정보

자료유형
학술저널
저자정보
Park, Hye-Bin (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) Park, Yeo-Reum (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) Lee, Hwa-Yeon (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) Bae, Hyo-Kyung (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) Lee, Seunghyung (College of Animal Life Sciences, Kangwon National University) Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University) Yang, Boo-Keun (College of Animal Life Sciences, Kangwon National University) Cheong, Hee-Tae (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
저널정보
한국동물번식학회 한국수정란이식학회지 한국수정란이식학회지 제32권 제1호
발행연도
2017.1
수록면
25 - 31 (7page)

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This study was conducted to investigate the effect of activation method on the endoplasmic reticulum (ER) stress induction, apoptosis and in vitro development of porcine parthenogenetic embryos. Porcine in vitro matured oocytes were activated by four activation methods; 1) electric stimulus (ES) (E), 2) $ES+10{\mu}M$ Ca-ionophore (A23187) treatment (EC), 3) ES+2 mM 6-dimethylaminopurine (6-DMAP) treatment (ED), or 4) ES+A23187 and 6-DMAP treatments (ECD). Parthenogenetic embryos were sampled to analyze x-box binding protein 1 (Xbp1) mRNA, ER stress-associated genes and apoptosis genes at 3 h after ES and the 1-cell and blastocyst stages. In the EC group, the band intensity of spliced Xbp1 (Xbp1s) mRNA was higher than those of the other groups at the 3 h and 1-cell stage, and higher than that of the E group at the blastocyst stage. Four ER stress-associated genes were expressed at the highest level in the EC group and weakly expressed in the ED group at 3 h after activation. However, most of the genes were highly expressed at the 1-cell and blastocyst stages with some variation in the EC and ECD groups. Expression of Bcl-2-associated X protein (Bax) and caspase-3 mRNA was significantly higher in the EC group than in the other groups at all development stages. The developmental rates to the blastocyst stage were higher in the ED and ECD groups than in the E and EC groups. These results suggest that the intracellular ER stress of parthenogenetic porcine embryos is affected by the activation method and subsequently lead to the apoptosis of embryos.

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