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자료유형
학술저널
저자정보
Sung, Gi-Ho (Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) Shrestha, Bhushan (Green Energy Mission/Nepal, Anam Nagar) Han, Sang-Kuk (Division of Forest Biodiversity, Korea National Arboretum) Kim, Soo-Young (Donghae Agricultural Technology Center) Sung, Jae-Mo (Cordyceps Institute of Mushtech)
저널정보
한국균학회 Mycobiology Mycobiology 제38권 제4호
발행연도
2010.1
수록면
274 - 281 (8page)

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Cordyceps cardinalis was reported in Japan and the USA in 2004, and its fruiting bodies have recently been cultured in Korea. Herbarium specimens preserved at the Cordyceps Research Institute, Mushtech, Korea were revised and identified as C. cardinalis, based on morphological characters and conidial structures. Most of the C. cardinalis specimens were collected from Mt. Halla in Jeju-do. The effects of various nutritional sources and environmental conditions such as temperature and pH on mycelial growth of C. cardinalis were studied. Oatmeal agar, Martin's peptone dextrose agar, and Schizophyllum (mushroom) genetics complete medium plus yeast extract resulted in the best mycelial growth. Among carbon sources, cereals, and nitrogen sources, maltose, oatmeal, and peptone resulted in the best mycelial growth respectively. Mineral salts helped to increase growth rate but only resulted in thin mycelial density, similar to water agar. A temperature of $25^{\circ}C$ and a pH of 7 resulted in the highest mycelial growth. Based on these results, a Cordyceps cardinalis composite medium (CCM) was formulated with 1% maltose, 2% oatmeal, 1% peptone, and 2% agar. Use of the CCM resulted in slightly better mycelial growth than that of other commonly used agar media. Only organic nitrogen sources imparted a reddish pigmentation to the agar media, but this character diminished after several subcultures. A 7 day culture duration resulted in the best mycelial growth.

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