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논문 기본 정보

자료유형
학술저널
저자정보
Wi, Hae-Joo (School of Veterinary Medicine and Kangwon National University) Kwon, Dae-Jin (School of Veterinary Medicine and Kangwon National University) Park, Joo-Hee (School of Veterinary Medicine and Kangwon National University) Park, Choon-Keun (College of Animal Resource Science, Kangwon National University) Yang, Boo-Keun (College of Animal Resource Science, Kangwon National University) Cheong, Hee-Tae (School of Veterinary Medicine and Kangwon National University)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제31권 제4호
발행연도
2007.1
수록면
273 - 278 (6page)

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This study was conducted to examine the protein kinase inhibitors, 6-dimethylaminopurine (DMAP) and cycloheximide (CHXM) on the development and chromosome constitution of porcine parthenogenetic embryos. In vitro matured oocytes were activated by electric stimuli (ES) or a combination of ES with culture in 2 mM DMAP or $10{\mu}g/ml$ CHXM for 4 hr. Activated oocytes were cultured in PZM-3 for 6 days. Some 1-cell embryos and blastocysts were fixed by air dry method to analyze the chromosome constitutions and/or total cell number. Blastocyst development of DMAP-treated group (26.7%) was significantly higher (p<0.05) than those of CHXM-treated and ES control groups. Ploidy in 1-cell stage embryos was not different among groups (77.3 to 81.0%), however, proportion of diploid chromosome constitutions was high in DMAP-treated group (61.9%, p<0.05). In the blastocyst stage, proportion of diploid chromosome plates was significantly high in DMAP-treated group (64.2%, p<0.05), and proportion of abnormal chromosome plates was higher in CHXM-treated group (36.6%, p<0.05) than DMAP-treated group (28.3%,). Proportion of embryos with abnormal chromosome constitutions was slightly increased by DMAP (40.0%) and CHXM (42.1%) treatment due to the increasing of mixoploid (47.4 and 52.0%). The present study shows that the DMAP treatment increase the development of porcine parthenotes. However, parthenogenetic activation by ES or combined treatment with ES and DMAP or CHXM detrimentally affects the chromosome constitutions of porcine parthenotes during early embryonic development, leads to increased abnormal ploidy in the blastocyst stage.

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