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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 813 - 820 (8page)
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NB4 cell, the human acute promyelocytic leukemia (APL) cell line, was treated with various concentrations of arsenic trioxide (ATO) to induce apoptosis, measured by staining with 7-amino-actinomycin D (7-AAD) by flow cytometry. 2’, 7’-dichlorodihydro-fluorescein-diacetate (DCF-DA) and MitoSOXTM Red mitochondrial superoxide indicator were used to detect intracellular and mitochondrial reactive oxygen species (ROS). The steady-state level of SO2 (Cysteine sulfinic acid, Cys?SO2H) form for peroxiredoxin 3 (PRX3) was measured by a western blot. To evaluate the effect of sulfiredoxin 1 depletion, NB4 cells were transfected with small interfering RNA and analyzed for their influence on ROS, redox enzymes, and apoptosis. The mitochondrial ROS of NB4 cells significantly increased after ATO treatment. NB4 cell apoptosis after ATO treatment increased in a time-dependent manner. Increased SO2 form and dimeric PRX3 were observed as a hyperoxidation reaction in NB4 cells post-ATO treatment, in concordance with mitochondrial ROS accumulation. Sulfiredoxin 1 expression is downregulated by small interfering RNA transfection, which potentiated mitochondrial ROS generation and cell growth arrest in ATO-treated NB4 cells. Our results indicate that ATO-induced ROS generation in APL cell mitochondria is attributable to PRX3 hyperoxidation as well as dimerized PRX3 accumulation, subsequently triggering apoptosis. The downregulation of sulfiredoxin 1 could amplify apoptosis in ATO-treated APL cells.
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참고문헌 (44)
참고문헌 신청
Agrawal-Singh, S. / 2012 / Genome-wide analysis of histone H3 acetylation patterns in AML identifies PRDX2 as an epigenetically silenced tumor suppressor gene / Blood 119:2346~2357
Bae, S. H. / 2012 / Peroxiredoxin III and sulfiredoxin together protect mice from pyrazole-induced oxidative liver injury / Antioxid. Redox Signal 17:1351~1361
Chou, W. C. / 2004 / Role of NADPH oxidase in arsenic-induced reactive oxygen species formation and cytotoxicity in myeloid leukemia cells / Proc. Natl. Acad. Sci. U. S. A 101:4578~4583
Coe, E. / 2008 / Catalase activity and arsenic sensitivity in acute leukemia / Leuk. Lymphoma 49:1976~1981
Cox, A. G. / 2010 / Mitochondrial peroxiredoxin involvement in antioxidant defence and redox signalling / Biochem. J 425:313~325
Bae, S. H. / 2012 / Peroxiredoxin III and sulfiredoxin together protect mice from pyrazole-induced oxidative liver injury / Antioxid. Redox Signal 17:1351~1361
Chou, W. C. / 2004 / Role of NADPH oxidase in arsenic-induced reactive oxygen species formation and cytotoxicity in myeloid leukemia cells / Proc. Natl. Acad. Sci. U. S. A 101:4578~4583
Coe, E. / 2008 / Catalase activity and arsenic sensitivity in acute leukemia / Leuk. Lymphoma 49:1976~1981
Cox, A. G. / 2010 / Mitochondrial peroxiredoxin involvement in antioxidant defence and redox signalling / Biochem. J 425:313~325
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