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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 1,179 - 1,188 (10page)
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Background There is now abundant information on genes involved in molluscan oogenesis and their associations with ovarian development. However, few studies have investigated the ovarian transcriptome of Pacifc abalone (Haliotis discus hannai). Objective The objective of this study was to identify genes related to ovarian development and maturation in Pacifc abalone utilizing RNA-sequencing (RNA-seq) and to verify the genes most relevant to diferent stages of maturation. Methods RNA samples from the ovarian tissues of sexually immature and mature abalone were used to construct cDNA libraries, which were paired-end sequenced on an Illumina HiSeq 2500 platform. Reads from individual samples (unigenes) were aligned to reference transcriptome databases for identifcation of diferentially expressed genes (DEGs) between immature and mature ovarian libraries. Reverse transcription-quantitative polymerase chain reaction was used to verify the RNA-seq data. Results A total of 8779 unigenes were obtained from the ovaries of immature and mature abalone, with a total length of 3323,279 bp and an average length of 379 bp per gene. Gene ontology analysis assigned 5860 unigenes to biological processes, 855 to cellular components, and 1352 to molecular functions. Overall, 470 DEGs were identifed, including 213 and 257 genes down-regulated and up-regulated in mature abalone, respectively. Among these, 13 relevant transcripts, including VTG1 and FZD7, were signifcantly highly expressed in the ovaries of mature abalone (p2). Conclusion This H. discus hannai ovary transcriptome provides molecular targets to better understand ovarian development, oogenesis, and sexual maturation, and to enhance Pacifc abalone production.
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참고문헌 (56)
참고문헌 신청
Andrews S / FastQC: a quality control tool for high throughput sequence data
Cao Z / 2018 / Comparative proteomic analysis of ovaries from Huoyan geese between pre-laying and laying periods using an iTRAQ-based approach / Poult Sci 97:2170~2182
Chen C / 2018 / Two distinct vitellogenin genes are similar in function and expression in the bigfin reef squid Sepioteuthis lessoniana / Biol Reprod 99:1034~1044
Cook PA / 2014 / The worldwide abalone industry / Mod Econ 5:1181~1186
Dheilly NM / 2012 / Gametogenesis in the Pacific oyster Crassostrea gigas : a microarrays-based analysis identifies sex and stage specific genes / PLoS ONE 7:e36353
Cao Z / 2018 / Comparative proteomic analysis of ovaries from Huoyan geese between pre-laying and laying periods using an iTRAQ-based approach / Poult Sci 97:2170~2182
Chen C / 2018 / Two distinct vitellogenin genes are similar in function and expression in the bigfin reef squid Sepioteuthis lessoniana / Biol Reprod 99:1034~1044
Cook PA / 2014 / The worldwide abalone industry / Mod Econ 5:1181~1186
Dheilly NM / 2012 / Gametogenesis in the Pacific oyster Crassostrea gigas : a microarrays-based analysis identifies sex and stage specific genes / PLoS ONE 7:e36353
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