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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 927 - 935 (9page)
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Background The main therapies for cancer often results in many side effects and drug resistance. Gamma linolenic acid (GLA) is a kind of natural reagent with negligible cytotoxicity. Objective This work aims at detecting whether GLA possesses anti-cancer activity in NSCLC cells and elucidating the potential molecular mechanism. Methods Cytotoxicity of GLA was evaluated by MTT assay and soft agar colony formation method. Immunoblotting analysis examined the effect of GLA on protein expressions of cell proliferation markers (e.g., PCNA, Ki-67 and MCM2), pro-survival protein bcl-2, apoptosis-associated proteins (e.g., bax and cleaved caspase 3), HIF1α and VEGF. Wound healing assay and transwell invasion assay were performed to test the effect of GLA on hypoxia-induced cell migration and invasion. Cell transfection was used to overexpress HIF1α followed by the treatment of GLA to test the effect of HIF1α overexpression on the tumoricidal activity of GLA in NSCLC cell lines. Results MTT and soft agar colony formation tests showed that GLA dose-dependently suppressed cell proliferation in both Calu-1 and SK-MES-1 cell lines. Immunoblotting analysis demonstrated that GLA suppressed protein expressions of PCNA, Ki-67, MCM2 and bcl-2, while GLA induced bax and cleaved caspase 3 expressions. Wound healing assay and transwell invasion assay revealed that GLA was very effective on the inhibition of NSCLC cell migration and invasion. Immunoblotting analysis and cell transfection method indicated that GLA inhibited hypoxia-induced cell proliferation and invasion by suppressing HIF1α-VEGF pathway. Conclusion GLA suppresses hypoxia-induced proliferation and invasion of NSCLC cells by inhibition of HIF1α pathway in vitro.
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참고문헌 (22)
참고문헌 신청
Abbott SK / 2012 / Fatty acid composition of membrane bilayers : importance of diet polyunsaturated fat balance / Biochim Biophys Acta 1818:1309~1317
Al Tameemi W / 2019 / Hypoxia-modified cancer cell metabolism / Front Cell Dev Biol 7:4
Colquhoun A / 2002 / Gamma-linolenic acid alters the composition of mitochondrial membrane subfractions, decreases outer mitochondrial membrane binding of hexokinase and alters carnitine palmitoyltransferase I properties in the Walker 256 rat tumour / Biochim Biophys Acta 1583:74~84
Das UN / 2011 / Effect of polyunsaturated fatty acids on drug-sensitive and resistant tumor cells in vitro / Lipids Health Dis 10:159~159
Ge H / 2013 / Gamma-linolenic acid induces apoptosis and lipid peroxidation in human chronic myelogenous leukemia K562 cells / Cell Biol Int 33:402~410
Al Tameemi W / 2019 / Hypoxia-modified cancer cell metabolism / Front Cell Dev Biol 7:4
Colquhoun A / 2002 / Gamma-linolenic acid alters the composition of mitochondrial membrane subfractions, decreases outer mitochondrial membrane binding of hexokinase and alters carnitine palmitoyltransferase I properties in the Walker 256 rat tumour / Biochim Biophys Acta 1583:74~84
Das UN / 2011 / Effect of polyunsaturated fatty acids on drug-sensitive and resistant tumor cells in vitro / Lipids Health Dis 10:159~159
Ge H / 2013 / Gamma-linolenic acid induces apoptosis and lipid peroxidation in human chronic myelogenous leukemia K562 cells / Cell Biol Int 33:402~410
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