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논문 기본 정보

자료유형
학술저널
저자정보
Nathalang Oytip (Thammasat University) Intharanut Kamphon (Thammasat University) Leetrakool Nipapan (Chiang Mai University) Mitundee Supattra (Thai Red Cross Society) Kupatawintu Pawinee (Thai Red Cross Society)
저널정보
대한혈액학회 Blood Research Blood Research Vol.55 No.2
발행연도
2020.1
수록면
107 - 111 (5page)

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초록· 키워드

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BackgroundSERF(+) is a high prevalence antigen in the Cromer blood group system that is encoded by a CROM*01.12 allele. The SERF(-) on red cells is caused by a single nucleotide variation, c.647C>T, in exon 5 of the Decay-accelerating factor, DAF gene. Alloanti-SERF was found in a pregnant Thai woman, and a SERF(-) individual was found among Thai blood donors. Since anti-SERF is commercially unavailable, this study aimed to develop appropriate gen-otyping methods for CROM*01.12 and CROM*01.-12 alleles and predict the SERF(-) phenotype in Thai blood donors.MethodsDNA samples obtained from 1,580 central, 300 northern, and 427 southern Thai blood donors were genotyped for CROM*01.12 and CROM*01.-12 allele detection using in-house PCR with sequence-specific primer (PCR-SSP) confirmed by DNA sequencing.ResultsValidity of the PCR-SSP genotyping results agreed with DNA sequencing; CROM*01.12/ CROM*01.12 was the most common (98.42%, 98.00%, and 98.59%), followed by CROM*01.12/CROM*01.-12 (1.58%, 2.00%, and 1.41%) among central, northern, and southern Thais, respectively. CROM*01.-12/CROM*01.-12 was not detected in all three populations. The alleles found in central Thais did not significantly differ from those found in northern and southern Thais. ConclusionThis study is the first to distinguish the predicted SERF phenotypes from genotyping re-sults obtained using in-house PCR-SSP, confirming that the CROM*01.-12 allele fre-quency ranged from 0.007 to 0.010 in three Thai populations. This helps identify the SERF(-) phenotype among donors and patients, ultimately preventing adverse transfusion reactions.

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