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Suraj Kumar (All India Institute of Medical Sciences New Delhi India) Sawan Bopanna (All India Institute of Medical Sciences New Delhi India) Saurabh Kedia (All India Institute of Medical Sciences New Delhi India) Pratap Mouli (All India Institute of Medical Sciences New Delhi India) Rajan Dhingra (All India Institute of Medical Sciences New Delhi India) Rajesh Padhan (All India Institute of Medical Sciences New Delhi India) Mikashmi Kohli (All India Institute of Medical Sciences New Delhi India) Jigyasa Chaubey (All India Institute of Medical Sciences New Delhi India) Rohini Sharma (All India Institute of Medical Sciences New Delhi India) Prasenjit Das (All India Institute of Medical Sciences New Delhi India) S Dattagupta (All India Institute of Medical Sciences New Delhi India) Govind Makharia (All India Institute of Medical Sciences New Delhi India) SK Sharma (All India Institute of Medical Sciences New Delhi India) Vineet Ahu (All India Institute of Medical Sciences New Delhi India)
저널정보
대한장연구학회 Intestinal research Intestinal research Vol.15 No.2
발행연도
2017.1
수록면
187 - 194 (8page)

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Background/Aims: The use of genetic probes for the diagnosis of pulmonary tuberculosis (TB) has been well described. However, the role of these assays in the diagnosis of intestinal tuberculosis is unclear. We therefore assessed the diagnostic utilityof the Xpert Mycobacterium tuberculosis /rifampicin (MTB/RIF) assay, and estimated the prevalence of multidrug-resistant(MDR) TB in the Indian population . Methods: Of 99 patients recruited, 37 had intestinal TB; two control groups comprised 43with Crohn’s disease (CD) and 19 with irritable bowel syndrome. Colonoscopy was performed before starting any therapy; mucosalbiopsies were subjected to histopathology, acid-fast bacilli staining, Lowenstein-Jensen culture, and nucleic acid amplificationtesting using the Xpert MTB/RIF assay. Patients were followed up for 6 months to confirm the diagnosis and responseto therapy. A composite reference standard was used for diagnosis of TB and assessment of the diagnostic utility of the XpertMTB/RIF assay. Results: Of 37 intestinal TB patients, the Xpert MTB/RIF assay was positive in three of 37 (8.1%), but none hadMDR-TB. The sensitivity, specificity, positive predictive value, and negative predictive value of the Xpert MTB/RIF assay was8.1%, 100%, 100%, and, 64.2%, respectively. Conclusions: The Xpert MTB/RIF assay has low sensitivity but high specificity forintestinal TB, and may be helpful in endemic tuberculosis areas, when clinicians are faced with difficulty differentiating TB andCD. Based on the Xpert MTB/RIF assay, the prevalence of intestinal MDR-TB is low in the Indian population.

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