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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 325 - 332 (8page)
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초록· 키워드
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Background and Objectives: Previously, various methodologies were used to enumerate the endothelial progenitor cells (EPCs). We now know that these methodologies enumerate at least three different EPC subsets: circulating angiogenic cells (CACs), colony-forming unit endothelial cells (CFU-ECs), and endothelial colony-forming cells (ECFCs). It is not clear whether there is a correlation between changes in the number of these subsets. The aim of the current study is to find an answer to this question. Materials and Methods: The number of all EPC subsets was quantified in the peripheral blood of nine pregnant women in their first and third trimesters of pregnancy. We enumerated 14 cell populations by quantitative flow-cytometry using various combinations of the markers, CD34, CD133, CD309, and CD45, to cover most of the reported phenotypes of CACs and ECFCs. Culturing technique was used to enumerate the CFU-ECs. Changes in the number of cells were calculated by subtracting the number of cells in the first trimester peripheral blood from the number of cells in the third trimester peripheral blood, and correlations between these changes were analyzed. Results: The number of CFU-ECs did not correlate with the number of ECFCs and CACs. Also, CACs and ECFCs showed independent behaviors. However, the number of CACs showed a strong correlation with the number of CD133+CD309+ cells (p=0.001) and a moderate correlation with the number of CD34+CD309+ cells (p=0.042). Also, the number of ECFCs was correlated with the number of CD309+CD45- cells (p=0.029) and CD34+CD45- cells (p=0.03). Conclusion: Our study showed that the three commonly used methods for quantifying EPC subsets represent different cells with independent behaviors. Also, any study that measured the number of EPCs using the flow cytometry method with a marker combination that lacks CD309 may be inaccurate.
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등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Attar A / 2013 / Colony forming unit endothelial cells do not exhibit telomerase alternative splicing variants and activity / Iran Biomed J 17:146~151
Asahara T / 1997 / Isolation of putative progenitor endothelial cells for angiogenesis / Science 275:964~967
Hill JM / 2003 / Circulating endothelial progenitor cells, vascular function, and cardiovascular risk / N Engl J Med 348:593~600
Lin C / 2009 / Maternal endothelial progenitor colony-forming units with macrophage characteristics are reduced in preeclampsia / Am J Hypertens 22:1014~1019
Peichev M / 20 / Expression of VEGFR-2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors / Blood 95:952~958
Asahara T / 1997 / Isolation of putative progenitor endothelial cells for angiogenesis / Science 275:964~967
Hill JM / 2003 / Circulating endothelial progenitor cells, vascular function, and cardiovascular risk / N Engl J Med 348:593~600
Lin C / 2009 / Maternal endothelial progenitor colony-forming units with macrophage characteristics are reduced in preeclampsia / Am J Hypertens 22:1014~1019
Peichev M / 20 / Expression of VEGFR-2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors / Blood 95:952~958
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