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Development of InDel Markers to Distinguish between Prunus mume, Prunus armeniaca var. ansu, and their Interspecific Hybrids
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매실나무와 살구나무, 종간잡종의 구별을 위한 InDel 마커의 개발

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Type
Academic journal
Author
Ju Hyeok Kim (충북대학교) Dong Wook Lee (충북대학교) Moon Kyo Kim (충북대학교) Mi Sun Lee (충북대학교) Nam Su Jo (충북대학교) Yi Lee (충북대학교)
Journal
The Korean Society of Medicinal Crop Science Korean Journal of Medicinal Crop Science Vol.30 No.3 KCI Excellent Accredited Journal SCOPUS
Published
2022.6
Pages
210 - 216 (7page)
DOI
10.7783/KJMCS.2022.30.3.210

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Development of InDel Markers to Distinguish between Prunus mume, Prunus armeniaca var. ansu, and their Interspecific Hybrids
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Background: Prunus mume Sieb. et. Zucc. is an important medicinal resource, and its fruit is used as a health food worldwide. However, this plant is often confused with Prunus armeniaca var. ansu., a related but distinct deciduous species. To distinguish between these plants, we developed co-dominant insertion-deletion (InDel) markers.
Methods and Results: DNA was extracted from P. mume and P. armeniaca leaves and subjected to next-generation sequencing (NGS) analysis. After assembly, 30 polymorphic loci were identified. Primers were designed for the polymorphic loci and used to genotype P. mume and P. armeniaca. Using two InDel markers developed from the polymorphic loci, we distinguished between P. mume and P. armeniaca, and also identified the genetic hybrids between P. mume and P. armeniaca.
Conclusions: The two markers developed in this study can be used to authenticate P. mume, P. armeniaca, and their hybrids.
#Prunus mume #Prunus armeniaca var. ansu #Genotyping #Insertion-Deletion #Next-Generation Sequencing

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