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학술저널
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이자랑 (한국생명공학연구원) 박상제 (한국생명공학연구원) 김영현 (한국생명공학연구원) 최세희 (한국생명공학연구원) 조현무 (한국생명공학연구원) 이상래 (한국생명공학연구원) 김선욱 (한국생명공학연구원) 김지수 (한국생명공학연구원) 심보웅 (한국생명공학연구원) 송봉석 (한국생명공학연구원) 정강진 (한국생명공학연구원) 이영전 (한국생명공학연구원) 진영배 (한국생명공학연구원) 강필용 (한국생명공학연구원) 허재원 (한국생명공학연구원) 장규태 (한국생명공학연구원)
저널정보
한국분자세포생물학회 Molecules and Cells Molecules and Cells 제40권 제2호
발행연도
2017.2
수록면
100 - 108 (9page)

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Cathepsin F, which is encoded by CTSF, is a cysteine proteinase ubiquitously expressed in several tissues. In a previous study, novel transcripts of the CTSF gene were identified in the crab-eating monkey deriving from the integration of an Alu element?AluYRa1. The occurrence of AluYRa1-derived alternative transcripts and the mechanism of exonization events in the CTSF gene of human, rhesus monkey, and crab-eating monkey were investigated using PCR and reverse transcription PCR on the genomic DNA and cDNA isolated from several tissues. Results demonstrated that AluYRa1 was only integrated into the genome of Macaca species and this lineage-specific integration led to exonization events by produc-ing a conserved 3’ splice site. Six transcript variants (V1?V6) were generated by alternative splicing (AS) events, including intron retention and alternative 5’ splice sites in the 5’ and 3’ flanking regions of CTSF_AluYRa1. Among them, V3?V5 transcripts were ubiquitously expressed in all tissues of rhesus monkey and crab-eating monkey, whereas AluYRa1-exonized V1 was dominantly expressed in the testis of the crab-eating monkey, and V2 was only expressed in the testis of the two monkeys. These five transcript variants also had different amino acid sequences in the C-terminal region of CTSF, as compared to reference sequences. Thus, species-specific Alu-derived exonization by lineage-specific integration of Alu elements and AS events seems to have played an important role during primate evolution by producing transcript variants and gene diversification.

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