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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.2
- 수록면
- 117 - 122 (6page)
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초록· 키워드
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Despite the fact that porcine embryonic stem cells (ESCs) are a practical study tool, in vitro long-term maintenance of these cells is difficult in a two-dimensional (2D) microenvironment using cellular niche or extracellular matrix proteins. However, a three-dimensional (3D) microenvironment, similar to that enclosing the inner cell mass of the blastocyst, may improve in vitro maintenance of self-renewal. Accordingly, as a first step toward constructing a 3D microenvironment optimized to maintain porcine ESC self-renewal, we investigated different culture dimensions for porcine ICM-derived cells to enhance the maintenance of self-renewal. Porcine ICM-derived cells were cultured in agarose-based 3D hydrogel with self-renewal-friendly mechanics and in 2D culture plates with or without feeder cells. Subsequently, the effects of the 3D microenvironment on maintenance of self-renewal were identified by analyzing colony formation and morphology, alkaline phosphatase (AP) activity, and transcriptional and translational regulation of self-renewal-related genes. The 3D microenvironment using a 1.5% (w/v) agarose-based 3D hydrogel resulted in significantly more colonies with stereoscopic morphology, significantly improved AP activity, and increased protein expression of self-renewal-related genes compared to those in the 2D microenvironment. These results demonstrate that self-renewal of porcine ICM-derived cells can be maintained more effectively in a 3D microenvironment than in a 2D microenvironment. These results will help develop novel culture systems for ICM-derived cells derived from diverse species, which will contribute to stimulating basic and applicable studies related to ESCs.
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참고문헌 (20)
참고문헌 신청
Baker, B. M. / 2012 / Deconstructing the third dimension:how 3D culture microenvironments alter cellular cues / J. Cell Sci 125:3015~3024
Dixon, J. E. / 2014 / Combined hydrogels that switch human pluripotent stem cells from selfrenewal to differentiation / Proc. Natl. Acad. Sci. USA 111:5580~5585
Donato, M. T. / 1999 / Characterization of drug metabolizing activities in pig hepatocytes for use in bioartificial liver devices: comparison with other hepatic cellular models / J. Hepatol 31:542~549
Gerecht, S. / 2007 / Hyaluronic acid hydrogel for controlled self-renewal and differentiation of human embryonic stem cells / Proc. Natl. Acad. Sci. USA 104:11298~11303
Hughes, G. C. / 2003 / Translational physiology: porcine models of human coronary artery disease:implications for preclinical trials of therapeutic angiogenesis / J. Appl. Physiol 94:1689~1701
Dixon, J. E. / 2014 / Combined hydrogels that switch human pluripotent stem cells from selfrenewal to differentiation / Proc. Natl. Acad. Sci. USA 111:5580~5585
Donato, M. T. / 1999 / Characterization of drug metabolizing activities in pig hepatocytes for use in bioartificial liver devices: comparison with other hepatic cellular models / J. Hepatol 31:542~549
Gerecht, S. / 2007 / Hyaluronic acid hydrogel for controlled self-renewal and differentiation of human embryonic stem cells / Proc. Natl. Acad. Sci. USA 104:11298~11303
Hughes, G. C. / 2003 / Translational physiology: porcine models of human coronary artery disease:implications for preclinical trials of therapeutic angiogenesis / J. Appl. Physiol 94:1689~1701
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