Long-term effects of human induced pluripotent stem cell-derived retinal cell transplantation in Pde6b knockout rats

Yang Jee Myung; Chung Sunho; Yun KyungA; Kim Bora; So Seongjun; Kang Seoon; Kang Eunju; Lee Joo Yong

doi:10.1038/s12276-021-00588-w

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자료유형: 학술저널

저자정보: Yang Jee Myung (University of Ulsan College of Medicine) Chung Sunho (University of Ulsan College of Medicine) Yun KyungA (University of Ulsan College of Medicine) Kim Bora (University of Ulsan College of Medicine) So Seongjun (University of Ulsan College of Medicine) Kang Seoon (University of Ulsan College of Medicine) Kang Eunju (University of Ulsan College of Medicine) Lee Joo Yong (University of Ulsan College of Medicine)

저널정보: 대한생화학·분자생물학회 Experimental and Molecular Medicine Experimental and Molecular Medicine 제53권

발행연도: 2021.4

수록면: 1 - 12 (12page)

DOI: 10.1038/s12276-021-00588-w

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Retinal degenerative disorders, including age-related macular degeneration and retinitis pigmentosa (RP), are characterized by the irreversible loss of photoreceptor cells and retinal pigment epithelial (RPE) cells; however, the long-term effect of implanting both human induced pluripotent stem cell (hiPSC)-derived RPE and photoreceptor for retinal regeneration has not yet been investigated. In this study, we evaluated the long-term effects of hiPSC-derived RPE and photoreceptor cell transplantation in Pde6b knockout rats to study RP; cells were injected into the subretinal space of the right eyes of rats before the appearance of signs of retinal degeneration at 2?3 weeks of age. Ten months after transplantation, we evaluated the cells using fundus photography, optical coherence tomography, and histological evaluation, and no abnormal cell proliferation was observed. A relatively large number of transplanted cells persisted during the first 4 months; subsequently, the number of these cells decreased gradually. Notably, immunohistochemical analysis revealed that the hiPSC-derived retinal cells showed characteristics of both RPE cells and photoreceptors of human origin after transplantation. Functional analysis of vision by scotopic electroretinogram revealed significant preservation of vision after transplantation. Our study suggests that the transplantation of hiPSC-derived retinal cells, including RPE cells and photoreceptors, has a potential therapeutic effect against irreversible retinal degenerative diseases.

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이 논문의 저자 정보

Yang Jee Myung

소속기관 Dongguk University Ilsan Hospital

주요연구분야 자연과학 > 화학

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Chung Sunho